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J Bacteriol. 1973 Sep;115(3):717-22. doi: 10.1128/jb.115.3.717-722.1973.
2
Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X-100.用曲拉通X-100溶解大肠杆菌的细胞质膜。
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本文引用的文献

1
A computer method for analysis of radioactivity data from single and double labeled experiments.一种用于分析单标记和双标记实验放射性数据的计算机方法。
Biochem Biophys Res Commun. 1971 May 21;43(4):717-22. doi: 10.1016/0006-291x(71)90674-7.
2
Some features of the bacterial membrane studied with the aid of a new fractionation technique.借助一种新的分级分离技术对细菌膜的一些特征进行了研究。
Biochem J. 1971 Apr;122(2):197-207. doi: 10.1042/bj1220197.
3
Isolation of a cell membrane-DNA-nascent RNA complex from bacteria.从细菌中分离细胞膜-DNA-新生RNA复合物。
J Mol Biol. 1969 Feb 28;40(1):65-76. doi: 10.1016/0022-2836(69)90296-4.
4
Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X-100.用曲拉通X-100溶解大肠杆菌的细胞质膜。
J Bacteriol. 1971 Oct;108(1):545-52. doi: 10.1128/jb.108.1.545-552.1971.
5
Lysis of the cell membrane of Escherichia coli K12 by ionic detergents.离子去污剂对大肠杆菌K12细胞膜的裂解作用。
Biochim Biophys Acta. 1970 Nov 12;224(1):288-90. doi: 10.1016/0005-2787(70)90650-7.
6
Examination of the protein composition of the cell envelope of Escherichia coli by polyacrylamide gel electrophoresis.通过聚丙烯酰胺凝胶电泳对大肠杆菌细胞膜的蛋白质组成进行检测。
J Bacteriol. 1970 Nov;104(2):882-9. doi: 10.1128/jb.104.2.882-889.1970.
7
The association of host and phage DNA with the membrane of Escherichia coli.宿主与噬菌体DNA与大肠杆菌细胞膜的关联。
Virology. 1970 Oct;42(2):420-36.
8
A mutation which changes a membrane protein of E. coli.一种改变大肠杆菌膜蛋白的突变。
Proc Natl Acad Sci U S A. 1969 Nov;64(3):957-61. doi: 10.1073/pnas.64.3.957.
9
Structure and biosynthesis of the bacterial cell wall.细菌细胞壁的结构与生物合成
Annu Rev Biochem. 1969;38:501-38. doi: 10.1146/annurev.bi.38.070169.002441.
10
Some unique properties of the deoxyribonucleic acid-bearing portion of the bacterial membrane.细菌细胞膜中携带脱氧核糖核酸部分的一些独特性质。
J Bacteriol. 1972 Oct;112(1):195-9. doi: 10.1128/jb.112.1.195-199.1972.

离子去污剂十二烷基肌氨酸钠对大肠杆菌细胞质膜的增溶作用。

Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate.

作者信息

Filip C, Fletcher G, Wulff J L, Earhart C F

出版信息

J Bacteriol. 1973 Sep;115(3):717-22. doi: 10.1128/jb.115.3.717-722.1973.

DOI:10.1128/jb.115.3.717-722.1973
PMID:4580564
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC246312/
Abstract

The sensitivity of the outer and cytoplasmic membranes of Escherichia coli to detergent was examined by isopycnic sucrose density gradient centrifugation. Sodium lauryl sarcosinate (Sarkosyl) was found to disrupt the cytoplasmic membrane selectively under conditions in which Triton X-100 and dodecyl sodium sulfate solubilized all membrane protein. These results were verified by gel electrophoresis; membrane proteins solubilized by Sarkosyl were identical to those of the cytoplasmic membrane. The presence of Mg(2+) during treatment with Sarkosyl was found to afford partial protection of the cytoplasmic membrane from dissolution.

摘要

通过等密度蔗糖密度梯度离心法检测了大肠杆菌外膜和细胞质膜对去污剂的敏感性。发现在Triton X-100和十二烷基硫酸钠溶解所有膜蛋白的条件下，月桂酰肌氨酸钠（Sarkosyl）能选择性地破坏细胞质膜。这些结果通过凝胶电泳得到了验证；Sarkosyl溶解的膜蛋白与细胞质膜的膜蛋白相同。发现在用Sarkosyl处理期间存在Mg(2+)可使细胞质膜得到部分保护而不被溶解。