离子去污剂十二烷基肌氨酸钠对大肠杆菌细胞质膜的增溶作用。
Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate.
作者信息
Filip C, Fletcher G, Wulff J L, Earhart C F
出版信息
J Bacteriol. 1973 Sep;115(3):717-22. doi: 10.1128/jb.115.3.717-722.1973.
Abstract
The sensitivity of the outer and cytoplasmic membranes of Escherichia coli to detergent was examined by isopycnic sucrose density gradient centrifugation. Sodium lauryl sarcosinate (Sarkosyl) was found to disrupt the cytoplasmic membrane selectively under conditions in which Triton X-100 and dodecyl sodium sulfate solubilized all membrane protein. These results were verified by gel electrophoresis; membrane proteins solubilized by Sarkosyl were identical to those of the cytoplasmic membrane. The presence of Mg(2+) during treatment with Sarkosyl was found to afford partial protection of the cytoplasmic membrane from dissolution.
摘要
通过等密度蔗糖密度梯度离心法检测了大肠杆菌外膜和细胞质膜对去污剂的敏感性。发现在Triton X-100和十二烷基硫酸钠溶解所有膜蛋白的条件下，月桂酰肌氨酸钠（Sarkosyl）能选择性地破坏细胞质膜。这些结果通过凝胶电泳得到了验证；Sarkosyl溶解的膜蛋白与细胞质膜的膜蛋白相同。发现在用Sarkosyl处理期间存在Mg(2+)可使细胞质膜得到部分保护而不被溶解。
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