Sambri V, Massaria F, Ardizzoni M, Stefanelli C, Cevenini R
Institute of Microbiology, University of Bologna, S. Orsola Hospital, Italy.
Zentralbl Bakteriol. 1993 Aug;279(3):330-5. doi: 10.1016/s0934-8840(11)80365-8.
The presence of glycoproteins in several Borrelia species was investigated by the digoxigenin labelling technique. The outer surface proteins A and B of seven isolates of the Lyme disease spirochete B. burgdorferi showed to be major glycosylated proteins. Few minor polypeptides with variable molecular masses were also present, at variance, in B. burgdorferi strains. Minor glycosylated proteins with varying molecular masses have been detected in the relapsing fever borreliae B. hermsii, B. turicatae and B. parkeri. B. turicatae showed also a major glycosylated protein with a molecular mass of approximately 40 kDa. Animal pathogenic borreliae B. anserina and B. coriaceae presented only minor glycosylated proteins with variable molecular masses.
采用地高辛标记技术研究了几种疏螺旋体中糖蛋白的存在情况。莱姆病螺旋体伯氏疏螺旋体的七个分离株的外表面蛋白A和B显示为主要的糖基化蛋白。不同的伯氏疏螺旋体菌株中还存在少数分子量可变的次要多肽。在回归热疏螺旋体赫氏疏螺旋体、土拉疏螺旋体和帕克疏螺旋体中检测到了分子量不同的次要糖基化蛋白。土拉疏螺旋体还显示出一种分子量约为40 kDa的主要糖基化蛋白。动物致病性疏螺旋体鹅疏螺旋体和科氏疏螺旋体仅呈现分子量可变的次要糖基化蛋白。
