Ohki K, Nagayama A, Nagata S
Osaka Bioscience Institute, Japan.
Growth Factors. 1991;5(3):183-9. doi: 10.3109/08977199109000282.
Mouse macrophage BAM3 cells produced colony-stimulating factors (CSFs) after stimulation with bacterial lipopolysaccharide (LPS). By assaying the CSF using various interleukin 3-dependent cell lines, it was shown that most of the CSFs produced by BAM3 cells were granulocyte CSF (G-CSF). The granulocyte-macrophage CSF (GM-CSF) gene was also expressed in BAM3 cells after stimulation with LPS. When BAM3 cells were fused with the mouse renal adenocarcinoma cell line RAG which does not produce G-CSF, two of four hybrid cell lines constitutively produced large quantities of G-CSF. About 300 bp of the promoter region of mouse G-CSF chromosomal gene was inserted upstream of the Escherichia coli chloramphenicol acetyltransferase gene, and introduced into BAM3, RAG and hybrid cells. The G-CSF promoter was activated by stimulation with LPS, in BAM3 cells, but was inert in RAG cells. On the other hand, there was significant constitutive CAT activity in the hybrid cells.
小鼠巨噬细胞BAM3细胞在受到细菌脂多糖（LPS）刺激后产生集落刺激因子（CSF）。通过使用各种依赖白细胞介素3的细胞系检测CSF，结果表明BAM3细胞产生的大多数CSF是粒细胞集落刺激因子（G-CSF）。在用LPS刺激后，粒细胞-巨噬细胞集落刺激因子（GM-CSF）基因也在BAM3细胞中表达。当BAM3细胞与不产生G-CSF的小鼠肾腺癌细胞系RAG融合时，四个杂交细胞系中的两个组成性地产生大量G-CSF。将小鼠G-CSF染色体基因启动子区域约300 bp插入大肠杆菌氯霉素乙酰转移酶基因的上游，并导入BAM3、RAG和杂交细胞中。在BAM3细胞中，G-CSF启动子通过LPS刺激而被激活，但在RAG细胞中无活性。另一方面，杂交细胞中有显著的组成性氯霉素乙酰转移酶（CAT）活性。
