Asano M, Nagata S
Osaka Bioscience Institute, Japan.
Gene. 1992 Nov 16;121(2):371-5. doi: 10.1016/0378-1119(92)90146-g.
The expression of the mouse gene (G-CSF) encoding granulocyte colony-stimulating factor is controlled by at least three regulatory elements, GPE1, GPE2 and GPE3 (G-CSF promoter elements). A set of 30-mer oligodeoxyribonucleotides (oligos) scanning the GPE3 region (-104 to -51) of the G-CSF promoter was synthesized, and the tetramer of each oligo was inserted upstream from the cat gene with the simian virus 40 enhancer element. By introducing these hybrid genes into human squamous carcinoma CHU-2 and mouse macrophage BAM3 cells, the enhancer core element of the GPE3 was localized to the region from -98 to -79 in the promoter. A nuclear factor which specifically binds to the core element of the GPE3 was constitutively detected in human CHU-2 cells, whereas the expression of a similar, but distinctly different, factor was significantly induced in BAM3 cells by lipopolysaccharide. The results suggest that these nuclear factors play important roles in the constitutive expression of G-CSF in CHU-2 cells and its inducible expression in macrophages.
编码粒细胞集落刺激因子的小鼠基因（G-CSF）的表达受至少三个调控元件GPE1、GPE2和GPE3（G-CSF启动子元件）的控制。合成了一组扫描G-CSF启动子GPE3区域（-104至-51）的30聚体寡脱氧核糖核苷酸（寡核苷酸），并将每个寡核苷酸的四聚体插入带有猿猴病毒40增强子元件的cat基因上游。通过将这些杂交基因导入人鳞状细胞癌CHU-2和小鼠巨噬细胞BAM3细胞，GPE3的增强子核心元件定位于启动子中-98至-79区域。在人CHU-2细胞中持续检测到一种与GPE3核心元件特异性结合的核因子，而脂多糖在BAM3细胞中显著诱导了一种相似但明显不同的因子的表达。结果表明，这些核因子在CHU-2细胞中G-CSF的组成性表达及其在巨噬细胞中的诱导性表达中起重要作用。
