乙醇增强鸡大脑皮层神经元中γ-氨基丁酸A型（GABAA）受体激活的氯离子电流。

Ethanol enhances GABAA receptor-activated chloride currents in chick cerebral cortical neurons.

作者信息

Reynolds J N, Prasad A

机构信息

Faculty of Medicine, Memorial University of Newfoundland, St. John's, Canada.

出版信息

Brain Res. 1991 Nov 8;564(1):138-42. doi: 10.1016/0006-8993(91)91363-6.

DOI:10.1016/0006-8993(91)91363-6

PMID:1723337

Abstract

Primary cultures of cerebral cortical neurons were prepared from 7- to 8-day-old chick embryos. The effect of ethanol on GABA-activated membrane current was examined using whole-cell voltage-clamp recording in cells maintained for 3-25 days in vitro. In approximately 60% of neurons examined ethanol caused a potentiation of the membrane current elicited by GABA. The threshold concentration of ethanol was 1 mM, and the potentiating effect of ethanol on GABA-activated currents was maximal at 10 mM. In many cells higher concentration (40-50 mM) of ethanol inhibited GABA-activated currents. These effects of ethanol were all reversible.

摘要

大脑皮质神经元的原代培养物取自7至8日龄的鸡胚。使用全细胞膜片钳记录技术，在体外培养3至25天的细胞中检测乙醇对γ-氨基丁酸（GABA）激活的膜电流的影响。在所检测的约60%的神经元中，乙醇使GABA引发的膜电流增强。乙醇的阈值浓度为1 mM，乙醇对GABA激活电流的增强作用在10 mM时最大。在许多细胞中，较高浓度（40 - 50 mM）的乙醇会抑制GABA激活的电流。乙醇的这些作用都是可逆的。