Windelborg Nielsen B, Lind P, Hansen B, Nansen P, Schiøtz P O
Department of Pediatrics, University Hospital of Aarhus, Denmark.
Clin Exp Allergy. 1991 Nov;21(6):725-32. doi: 10.1111/j.1365-2222.1991.tb03202.x.
Histamine release (HR) from washed human blood cells after challenge with the excretory-secretory antigens (ES) of the parasitic nematodes Toxocara canis and Ascaris suum was studied, employing a recently developed microfibre-based assay combined with hyperosmolar release media for maximal sensitivity. Blood samples were obtained from 30 patients suspected of parasite infection and 11 healthy volunteers serving as controls. Specific antibodies of IgE, IgG1 and IgG4 subclasses were determined by ELISA. Virtually no HR could be provoked by ES in the 11 controls. In contrast, HR was seen in 16 patients after challenge with T. canis ES and in 11 patients with A. suum ES. In the majority of these, HR was detectable after challenge with ES protein concentrations of less than 1 ng/ml, and the maximal HR obtained with ES was greater than that seen with optimal concentrations of anti-IgE. The HR after ES antigen challenge correlated with the amount of specific IgE in patient plasma, and coincided with the presence of specific IgG1 and IgG4.
采用最近开发的基于微纤维的检测方法并结合高渗释放介质以获得最大灵敏度，研究了用犬弓首蛔虫和猪蛔虫这两种寄生线虫的排泄-分泌抗原（ES）刺激后，洗涤过的人血细胞中的组胺释放（HR）情况。从30名疑似寄生虫感染的患者和11名作为对照的健康志愿者身上采集血样。通过酶联免疫吸附测定法（ELISA）测定IgE、IgG1和IgG4亚类的特异性抗体。在11名对照者中，ES几乎不能诱发HR。相比之下，在用犬弓首蛔虫ES刺激后，16名患者出现了HR，在用猪蛔虫ES刺激后，11名患者出现了HR。在大多数这些患者中，用浓度低于1 ng/ml的ES蛋白刺激后可检测到HR，并且用ES获得的最大HR大于用最佳浓度的抗IgE所观察到的HR。ES抗原刺激后的HR与患者血浆中特异性IgE的量相关，并且与特异性IgG1和IgG4的存在一致。
