Váli László, Stefanovits-Bányai Eva, Szentmihályi Klára, Fébel Hedvig, Sárdi Eva, Lugasi Andrea, Kocsis Ibolya, Blázovics Anna
II Department of Medicine, Semmelweis University, Budapest, Hungary.
Nutrition. 2007 Feb;23(2):172-8. doi: 10.1016/j.nut.2006.11.004.
Table beet (Beta vulgaris var. rubra) contains important bioactive agents (betaine and polyphenols), which have a wide range of physiologic effects. Because nutritive antioxidants may reduce the occurrence of complications and postoperative mortality, dietary intake of polyphenols and vitamins before surgery may greatly contribute to the survival of patients. Our aim was to determine the liver-protecting properties of bioactive substances of table beet in a model of ischemia-reperfusion injury of the rat.
Wistar rats were divided into two groups: non-treated (n = 24) and fed with table beet (n = 8). For 10 days the second group was treated with lyophilized table beet (2 g/kg body weight daily) mixed into the rat chow. Hepatic ischemia was maintained for 45 min, followed by 15 min of reperfusion. Ischemia-reperfusion was carried out on animals from both groups. Chemiluminescent intensity, H-donating ability, reducing power, free SH group concentration, Randox-total antioxidant status, glutathione peroxidase, and superoxide dismutase activities were determined by luminometry and spectrophotometry. Fatty acid (Shimadzu GC) and metal ion (inductively coupled plasma optical emission spectrometry) concentrations were observed in the liver.
As a result of feeding, global parameters (H-donating ability, reducing power, free SH group concentration) and enzymatic antioxidants (glutathione peroxidase and superoxide dismutase) of the liver were found to increase significantly, which indicated that the treatment had a positive effect on its redox state. The increase found in zinc and copper content may protect the hepatocytes against oxidative stress because these elements are required for the function of superoxide dismutase enzymes. In the table beet group the concentration of short-chain fatty acids decreased, whereas that of long-chain fatty acids increased. The changes in metal element and fatty acid concentrations confirmed that these elements have an essential function in cellular pathways.
It may be stated that a natural antioxidant-rich diet has a positive effect on redox homeostasis during hepatic ischemia-reperfusion.
红甜菜（Beta vulgaris var. rubra）含有重要的生物活性物质（甜菜碱和多酚），具有广泛的生理作用。由于营养性抗氧化剂可能会减少并发症的发生和术后死亡率，术前饮食中摄入多酚和维生素可能对患者的生存有很大帮助。我们的目的是在大鼠缺血再灌注损伤模型中确定红甜菜生物活性物质的肝脏保护特性。
将Wistar大鼠分为两组：未处理组（n = 24）和喂食红甜菜组（n = 8）。第二组大鼠连续10天喂食混入大鼠饲料中的冻干红甜菜（每日2 g/kg体重）。肝脏缺血持续45分钟，随后再灌注15分钟。对两组动物均进行缺血再灌注。通过发光法和分光光度法测定化学发光强度、氢供体能力、还原能力、游离巯基浓度、兰多克斯总抗氧化状态、谷胱甘肽过氧化物酶和超氧化物歧化酶活性。观察肝脏中的脂肪酸（岛津气相色谱仪）和金属离子（电感耦合等离子体发射光谱法）浓度。
喂食后发现，肝脏的整体参数（氢供体能力、还原能力、游离巯基浓度）和酶促抗氧化剂（谷胱甘肽过氧化物酶和超氧化物歧化酶）显著增加，这表明该处理对其氧化还原状态有积极影响。锌和铜含量的增加可能保护肝细胞免受氧化应激，因为这些元素是超氧化物歧化酶发挥功能所必需的。在红甜菜组中，短链脂肪酸浓度降低，而长链脂肪酸浓度增加。金属元素和脂肪酸浓度的变化证实了这些元素在细胞途径中具有重要作用。
可以说，富含天然抗氧化剂的饮食对肝脏缺血再灌注期间的氧化还原稳态有积极影响。
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