Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China (mainland).
Research Division of Clinical Pharmacology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China (mainland).
Med Sci Monit. 2017 Nov 17;23:5472-5479. doi: 10.12659/msm.905168.
BACKGROUND Delayed graft function (DGF) is a common complication that impairs allograft function after kidney transplantation. However, the mechanism of DGF remains unclear. Nuclear magnetic resonance (NMR)-based analysis has been widely used in recent times to assess changes in metabolite levels. MATERIAL AND METHODS Samples of perfusate from allografts donated after circulatory death were collected prior to transplantation, during static cold storage. ¹H-NMR-based metabolomics combined with the statistical methods, orthogonal partial least-squares discriminant analysis (OPLS-DA), and principle-component analysis (PCA), were employed to test different levels of metabolites between the allografts that exhibited DGF and those that exhibited immediate graft function (IGF). RESULTS The study population consisted of 36 subjects, 11 with DGF and 25 with IGF. Of the 37 detected and identified metabolites, a-glucose and citrate were significantly elevated in the perfusate of DGF allografts, and taurine and betaine were significantly decreased. CONCLUSIONS ¹H-NMR analysis of DGF and IGF perfusates revealed some significant differences in their metabolite profiles, which may help explain the mechanisms of kidney ischemia-reperfusion injury and DGF.
延迟性肾功能恢复（DGF）是一种常见的并发症，会损害肾移植后移植物的功能。然而，DGF 的发生机制尚不清楚。基于核磁共振（NMR）的分析在最近得到了广泛应用，用于评估代谢物水平的变化。
在移植前、静态冷储存期间，收集来自循环死亡后捐献的供体的灌流液样本。采用基于 ¹H-NMR 的代谢组学结合统计学方法正交偏最小二乘判别分析（OPLS-DA）和主成分分析（PCA），测试表现出 DGF 和立即移植物功能（IGF）的供体移植物之间不同水平的代谢物。
研究人群包括 36 名受试者，其中 11 名患有 DGF，25 名患有 IGF。在检测和鉴定的 37 种代谢物中，DGF 供体灌流液中的 a-葡萄糖和柠檬酸明显升高，牛磺酸和甜菜碱明显降低。
对 DGF 和 IGF 灌流液的 ¹H-NMR 分析显示，它们的代谢物图谱存在一些显著差异，这可能有助于解释肾缺血再灌注损伤和 DGF 的发生机制。
