Beveridge T J, Graham L L
Department of Microbiology, College of Biological Science, University of Guelph, Ontario, Canada.
Microbiol Rev. 1991 Dec;55(4):684-705. doi: 10.1128/mr.55.4.684-705.1991.
Since bacteria are so small, microscopy has traditionally been used to study them as individual cells. To this end, electron microscopy has been a most powerful tool for studying bacterial surfaces; the viewing of macromolecular arrangements of some surfaces is now possible. This review compares older conventional electron-microscopic methods with new cryotechniques currently available and the results each has produced. Emphasis is not placed on the methodology but, rather, on the importance of the results in terms of our perception of the makeup and function of bacterial surfaces and their interaction with the surrounding environment.
由于细菌非常小，传统上一直使用显微镜将它们作为单个细胞进行研究。为此，电子显微镜一直是研究细菌表面的最强大工具；现在已经能够观察到一些表面的大分子排列。本综述将传统的电子显微镜方法与目前可用的新冷冻技术及其各自产生的结果进行了比较。重点不在于方法，而是在于这些结果对于我们理解细菌表面的组成、功能及其与周围环境的相互作用的重要性。
