Talou J R, Cascone O, Giulietti A M
Cátedra de Microbiología Industrial y Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, (1113) Junín 956, Buenos Aires, Argentina.
Planta Med. 1994 Jun;60(3):260-2. doi: 10.1055/s-2006-959471.
The presence of thiophenes in four Argentinian species of TAGETES was studied. T. TERNIFLORA HBK and T. MINUTA L. seedlings contain 5-(4-hydroxy-1-butynyl)-2-2'-bithienyl (BBTOH); 5-(4-acetoxy-1-butynyl)-2,2'-bithienyl (BBTOAc), while T. CAMPANULATA Griseb and T. LAXA Cabrera seedlings also accumulated BBT and alpha-T. From the four TAGETES species tested only T. LAXA was able to produce transformed roots when infected with AGROBACTERIUM RHIZOGENES LBA 9402. Several clones of transformed roots were obtained in which the total thiophene content present showed considerable variations (277 to 1773 microg/g FW). The thiophene spectrum, however, was similar between different clones. In addition, the thiophene patterns in these transformed clones differed from that formed in the parent plants.
对阿根廷四种万寿菊属植物中噻吩的存在情况进行了研究。三裂叶万寿菊（Tagetes terniflora HBK）和细叶万寿菊（T. minima L.）的幼苗含有5-(4-羟基-1-丁炔基)-2,2'-联噻吩（BBTOH）；5-(4-乙酰氧基-1-丁炔基)-2,2'-联噻吩（BBTOAc），而钟花万寿菊（T. campanulata Griseb）和疏花万寿菊（T. laxa Cabrera）的幼苗也积累了BBT和α-T。在所测试的四种万寿菊属植物中，只有疏花万寿菊在被发根农杆菌（Agrobacterium rhizogenes）LBA 9402感染时能够产生转化根。获得了几个转化根克隆，其中总噻吩含量显示出相当大的差异（277至1773微克/克鲜重）。然而，不同克隆之间的噻吩谱相似。此外，这些转化克隆中的噻吩模式与亲本植物中形成的模式不同。
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