NOVAPLANT Cell Biotechnology Group, Department of Experimental Botany, University of Nijmegen, Toernooiveld, NL-6525 ED, Nijmegen, The Netherlands.
Planta. 1989 Aug;179(1):43-50. doi: 10.1007/BF00395769.
Roots of marigold (Tagetes patula L.) accumulate thiophenes, heterocyclic sulfurous compounds with strong biocidal activity. In detached roots cultured in vitro, the thiophene content was 5 μmol·(g fresh weight)(-1) which is 25-times higher than in roots attached to the plant. In roots derived from tissues transformed by Agrobacterium tumefaciens and A. rhizogenes, the morphology and thiophene content varied with the bacterial strain used. Transformation stimulated the elongation of the root tips and the formation of lateral roots but lowered the thiophene level to 20-50% relative to the concentration in untransformed detached roots. A negative correlation was found between the number of laterals in a root system and the thiophene content. Extensive branching and a decrease in thiophene accumulation was evoked in untransformed roots by indole-3-acetic acid (1-10 μmol·l(-1)) added to the medium. Within the roots, the highest thiophene concentrations were found in the tips. The results indicate that auxin directly or indirectly plays a role in the regulation of the thiophene level in root tips.
万寿菊花（Tagetes patula L.）的根部积累了噻吩，这是一种具有强烈杀菌活性的杂环含硫化合物。在离体培养的根系中，噻吩含量为 5 μmol·(g 鲜重)(-1)，是附着在植物上根系的 25 倍。在根组织经根癌农杆菌和发根农杆菌转化后，噻吩含量和形态因细菌菌株的不同而有所差异。转化刺激根尖的伸长和侧根的形成，但将噻吩水平降低到未转化的离体根系浓度的 20-50%。根系中侧根的数量与噻吩含量呈负相关。吲哚-3-乙酸（1-10 μmol·l(-1))添加到培养基中会引起未转化的根系广泛分枝和噻吩积累减少。在根系中，噻吩浓度最高的部位是根尖。这些结果表明，生长素直接或间接地在调节根尖噻吩水平方面发挥作用。
