利用基于纤维的荧光相关光谱法检测β-淀粉样蛋白聚集
Detecting amyloid-beta aggregation with fiber-based fluorescence correlation spectroscopy.
作者信息
Garai Kanchan, Sureka Ruchi, Maiti S
出版信息
Biophys J. 2007 Apr 1;92(7):L55-7. doi: 10.1529/biophysj.106.101485. Epub 2007 Jan 19.
Abstract
Soluble aggregates critically influence the chemical and biological aspects of amyloid protein aggregation, but their population is difficult to measure, especially in vivo. We take an optical fiber-based fluorescence correlation spectroscopy (FCS) approach to characterize a solution of aggregating amyloid-beta molecules. We find that this technique can easily resolve aggregate particles of size 100 nm or greater in vitro, and the size distribution of these particles agrees well with that obtained by conventional FCS techniques. We propose fiber FCS as a tool for studying aggregation in vivo.
摘要
可溶性聚集体对淀粉样蛋白聚集的化学和生物学方面有着至关重要的影响，但其数量难以测量，尤其是在体内。我们采用基于光纤的荧光相关光谱法（FCS）来表征聚集的β-淀粉样蛋白分子溶液。我们发现，该技术能够轻松分辨体外大小为100纳米或更大的聚集颗粒，并且这些颗粒的大小分布与通过传统FCS技术获得的结果吻合良好。我们提出将光纤FCS作为一种研究体内聚集的工具。
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