• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

朊病毒样蛋白聚集的表征：新兴的基于纳米孔的方法。

Characterizing Prion-Like Protein Aggregation: Emerging Nanopore-Based Approaches.

作者信息

Meyer Nathan, Torrent Joan, Balme Sébastien

机构信息

Institut Européen des Membranes, UMR5635 University of Montpellier ENCSM CNRS, Place Eugène Bataillon, Cedex 5, Montpellier, 34095, France.

INM, University of Montpellier, INSERM, Montpellier, 34095, France.

出版信息

Small Methods. 2024 Dec;8(12):e2400058. doi: 10.1002/smtd.202400058. Epub 2024 Apr 21.

DOI:10.1002/smtd.202400058
PMID:38644684
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11672191/
Abstract

Prion-like protein aggregation is characteristic of numerous neurodegenerative diseases, such as Alzheimer's and Parkinson's diseases. This process involves the formation of aggregates ranging from small and potentially neurotoxic oligomers to highly structured self-propagating amyloid fibrils. Various approaches are used to study protein aggregation, but they do not always provide continuous information on the polymorphic, transient, and heterogeneous species formed. This review provides an updated state-of-the-art approach to the detection and characterization of a wide range of protein aggregates using nanopore technology. For each type of nanopore, biological, solid-state polymer, and nanopipette, discuss the main achievements for the detection of protein aggregates as well as the significant contributions to the understanding of protein aggregation and diagnostics.

摘要

朊病毒样蛋白聚集是许多神经退行性疾病的特征，如阿尔茨海默病和帕金森病。这个过程涉及从小的、可能具有神经毒性的寡聚体到高度结构化的自我传播淀粉样纤维的聚集体形成。人们使用了各种方法来研究蛋白质聚集，但它们并不总是能提供关于所形成的多态性、瞬态和异质物种的连续信息。本综述提供了一种最新的先进方法，用于使用纳米孔技术检测和表征各种蛋白质聚集体。对于每种类型的纳米孔，包括生物纳米孔、固态聚合物纳米孔和纳米吸管，讨论了检测蛋白质聚集体的主要成果以及对理解蛋白质聚集和诊断的重大贡献。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd81/11672191/7b459f382001/SMTD-8-2400058-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd81/11672191/f77e75d39d4c/SMTD-8-2400058-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd81/11672191/64cebde74791/SMTD-8-2400058-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd81/11672191/1b2c9e9b76a0/SMTD-8-2400058-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd81/11672191/df875e820330/SMTD-8-2400058-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd81/11672191/506b4927e1b0/SMTD-8-2400058-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd81/11672191/a44d6baa517d/SMTD-8-2400058-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd81/11672191/7b459f382001/SMTD-8-2400058-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd81/11672191/f77e75d39d4c/SMTD-8-2400058-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd81/11672191/64cebde74791/SMTD-8-2400058-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd81/11672191/1b2c9e9b76a0/SMTD-8-2400058-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd81/11672191/df875e820330/SMTD-8-2400058-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd81/11672191/506b4927e1b0/SMTD-8-2400058-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd81/11672191/a44d6baa517d/SMTD-8-2400058-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd81/11672191/7b459f382001/SMTD-8-2400058-g005.jpg

相似文献

1
Characterizing Prion-Like Protein Aggregation: Emerging Nanopore-Based Approaches.朊病毒样蛋白聚集的表征：新兴的基于纳米孔的方法。
Small Methods. 2024 Dec;8(12):e2400058. doi: 10.1002/smtd.202400058. Epub 2024 Apr 21.
2
Investigating the Aggregation and Prionogenic Properties of Human Cancer-Related Proteins.研究人类癌症相关蛋白的聚集及朊病毒生成特性。
Mol Cell Biol. 2025;45(4):154-168. doi: 10.1080/10985549.2025.2481054. Epub 2025 Mar 31.
3
Aerolysin Nanopore Electrochemistry.气单胞菌溶素纳米孔电化学
Acc Chem Res. 2025 Feb 18;58(4):517-528. doi: 10.1021/acs.accounts.4c00630. Epub 2025 Jan 28.
4
Short-Term Memory Impairment短期记忆障碍
5
Chiral nanomaterial-based approaches for diagnosis and treatment of protein-aggregated neurodiseases: current status and future opportunities.基于手性纳米材料的用于诊断和治疗蛋白聚集性神经疾病的方法：现状和未来机遇。
J Mater Chem B. 2024 Feb 21;12(8):1991-2005. doi: 10.1039/d3tb02381h.
6
A rapid and systematic review of the clinical effectiveness and cost-effectiveness of paclitaxel, docetaxel, gemcitabine and vinorelbine in non-small-cell lung cancer.对紫杉醇、多西他赛、吉西他滨和长春瑞滨在非小细胞肺癌中的临床疗效和成本效益进行的快速系统评价。
Health Technol Assess. 2001;5(32):1-195. doi: 10.3310/hta5320.
7
An expanded method for malaria parasite genetic surveillance using targeted nanopore sequencing.一种使用靶向纳米孔测序进行疟原虫基因监测的扩展方法。
Gates Open Res. 2025 Jul 24;9:49. doi: 10.12688/gatesopenres.16355.1. eCollection 2025.
8
Formation of an Amyloid-like Structure During In Vitro Interaction of Titin and Myosin-Binding Protein C.肌联蛋白与肌球蛋白结合蛋白C体外相互作用过程中类淀粉样结构的形成
Int J Mol Sci. 2025 Jul 18;26(14):6910. doi: 10.3390/ijms26146910.
9
TDP43 and huntingtin Exon-1 undergo a conformationally specific interaction that strongly alters the fibril formation of both proteins.TDP43 和亨廷顿外显子 1 发生构象特异性相互作用，强烈改变这两种蛋白质的纤维形成。
J Biol Chem. 2024 Sep;300(9):107660. doi: 10.1016/j.jbc.2024.107660. Epub 2024 Aug 13.
10
An aminosterol breaks the autocatalytic cycle of Aβ aggregation and protects cell membranes from its soluble aggregates.一种氨基甾醇打破了Aβ聚集的自催化循环，并保护细胞膜免受其可溶性聚集体的影响。
Proc Natl Acad Sci U S A. 2025 Jul 15;122(28):e2417944122. doi: 10.1073/pnas.2417944122. Epub 2025 Jul 7.

引用本文的文献

1
Wafer-scale fabrication of solid-state nanopore array with a novel SpacerX process.采用新型SpacerX工艺进行固态纳米孔阵列的晶圆级制造。
Microsyst Nanoeng. 2025 Jun 25;11(1):129. doi: 10.1038/s41378-025-00979-3.
2
Detection of protein oligomers with nanopores.利用纳米孔检测蛋白质寡聚体。
Nat Rev Chem. 2025 Apr;9(4):224-240. doi: 10.1038/s41570-025-00694-7. Epub 2025 Mar 5.

本文引用的文献

1
Influence of Seed structure on Volume distribution of α-Synuclein Oligomer at Early Stages of Aggregation using nanopipette.使用纳米移液器研究种子结构对α-突触核蛋白寡聚体聚集早期体积分布的影响。
Chembiochem. 2024 Mar 1;25(5):e202300748. doi: 10.1002/cbic.202300748. Epub 2024 Feb 2.
2
Aggregation and Oligomerization Characterization of ß-Lactoglobulin Protein Using a Solid-State Nanopore Sensor.利用固态纳米孔传感器对β-乳球蛋白蛋白的聚集和寡聚化特性进行表征。
Sensors (Basel). 2023 Dec 22;24(1):81. doi: 10.3390/s24010081.
3
Multiplexed Digital Characterization of Misfolded Protein Oligomers via Solid-State Nanopores.
通过固态纳米孔对错误折叠蛋白寡聚物进行多重数字特征分析。
J Am Chem Soc. 2023 Nov 29;145(47):25776-25788. doi: 10.1021/jacs.3c09335. Epub 2023 Nov 16.
4
Single-Molecule Detection of α-Synuclein Oligomers in Parkinson's Disease Patients Using Nanopores.利用纳米孔对帕金森病患者的α-突触核蛋白寡聚物进行单分子检测。
ACS Nano. 2023 Nov 28;17(22):22999-23009. doi: 10.1021/acsnano.3c08456. Epub 2023 Nov 10.
5
Patterning amyloid-β aggregation under the effect of acetylcholinesterase using a biological nanopore - an study.利用生物纳米孔在乙酰胆碱酯酶作用下对淀粉样β蛋白聚集进行模式化研究
Sens Actuators Rep. 2023 Dec;6. doi: 10.1016/j.snr.2023.100170. Epub 2023 Jul 8.
6
Ultrasensitive Detection of Aβ42 Seeds in Cerebrospinal Fluid with a Nanopipette-Based Real-Time Fast Amyloid Seeding and Translocation Assay.基于纳米移液器的实时快速淀粉样蛋白成核和转位分析的脑脊液中 Aβ42 种子的超高灵敏度检测。
Anal Chem. 2023 Aug 29;95(34):12623-12630. doi: 10.1021/acs.analchem.3c00017. Epub 2023 Aug 16.
7
Combining iontronic, chromatography and nanopipette for Aβ42 aggregates detection and separation.结合离子电子学、色谱法和纳米吸管用于检测和分离β淀粉样蛋白42聚集体。
Anal Chim Acta. 2023 Sep 22;1275:341587. doi: 10.1016/j.aca.2023.341587. Epub 2023 Jul 4.
8
Aβ42 fibril and non-fibril oligomers characterization using a nanopipette.利用纳米移液器对 Aβ42 纤维和无纤维寡聚物进行表征。
Biophys Chem. 2023 Sep;300:107076. doi: 10.1016/j.bpc.2023.107076. Epub 2023 Jul 17.
9
Discrimination between Alpha-Synuclein Protein Variants with a Single Nanometer-Scale Pore.利用单纳米尺度孔区分α-突触核蛋白变体。
ACS Chem Neurosci. 2023 Jul 19;14(14):2517-2526. doi: 10.1021/acschemneuro.3c00164. Epub 2023 Jun 29.
10
Simultaneous Determination of the Size and Shape of Single α-Synuclein Oligomers in Solution.溶液中单 α-突触核蛋白寡聚物的大小和形状的同时测定。
ACS Nano. 2023 Jul 11;17(13):12325-12335. doi: 10.1021/acsnano.3c01393. Epub 2023 Jun 16.