Schulte E K, Lyon H, Prento P
Department of Anatomy, University of Munich, Germany.
Histochem J. 1991 May;23(5):241-5. doi: 10.1007/BF01462247.
In the present study, the staining characteristics of the Gallocyanin chromalum technique devised by Husain and Watts are compared with the Feulgen reaction. Liver imprints, blood smears, and cervical smears were fixed in ethanol and stained with either the Husain and Watts Gallocyanin chromalum reagent or the Feulgen-Schiff reagent. The slides were then post-treated with 70% ethanol-HCl pH 1.0, or with phosphotungstic acid for 0.5-30 min. The integrated optical density of cell nuclei was measured with a VIDAS image analyzer. In the material stained with the Husain and Watts procedure, some Gallocyanin chromalum was removed from the nuclei in the early phase (5 min) of all the post-treatment steps, followed by a plateau phase where the integrated optical density remained constant for 30 min. In this phase, the nuclear absorbance was highly reproducible and of the same size regardless of the post-treatment. Both the Husain and Watts procedure and the Feulgen-reaction gave quantitative staining of DNA. The Gallocyanin chromalum stain after Husain and Watts is a quick staining procedure for quantitative evaluation of DNA in cytological material. Proper rinsing of the slides is necessary for a good reproducibility of results.
在本研究中，将胡赛因和瓦茨设计的间苯三酚铬明矾技术的染色特性与福尔根反应进行了比较。肝脏压片、血涂片和宫颈涂片用乙醇固定，并用胡赛因和瓦茨间苯三酚铬明矾试剂或福尔根-席夫试剂染色。然后将玻片用pH 1.0的70%乙醇-HCl或磷钨酸后处理0.5 - 30分钟。用VIDAS图像分析仪测量细胞核的积分光密度。在用胡赛因和瓦茨方法染色的材料中，在所有后处理步骤的早期阶段（5分钟），一些间苯三酚铬明矾从细胞核中被去除，随后是一个平台期，在此期间积分光密度保持30分钟恒定。在此阶段，无论后处理如何，细胞核吸光度都具有高度可重复性且大小相同。胡赛因和瓦茨方法以及福尔根反应都能对DNA进行定量染色。胡赛因和瓦茨的间苯三酚铬明矾染色是一种对细胞学材料中的DNA进行定量评估的快速染色方法。为了获得良好的结果重现性，对玻片进行适当冲洗是必要的。
