Schulte E, Wittekind D
Department of Anatomy, University of Freiburg, Federal Republic of Germany.
Anal Quant Cytol Histol. 1990 Jun;12(3):149-56.
The staining characteristics of five nuclear stains used in a Papanicolaou staining procedure were investigated. Alcohol-fixed cervical smears were stained with a modified Papanicolaou procedure using hematoxylin, alcoholic thionin bromide, alcoholic Victoria blue B, gallocyanin or the thionin Feulgen reagent (thionin-SO2) as the nuclear stain. The same anionic counterstain was used for all slides, and the optical densities of cell nuclei and cytoplasm were measured with the IBAS 2000 image analyzer. Alcoholic thionin gave the most intense nuclear stain, with a very high reproducibility of the staining pattern. Hematoxylin showed the highest coefficient of variation of the staining intensity. Both hematoxylin and gallocyanin gave some nonspecific cytoplasmic staining. Thionin-SO2 allowed a quantitative assessment of DNA, but gave a low staining intensity. Staining with the metal complex dyes interfered with subsequent staining with the pararosaniline Feulgen reagent. Alcoholic thioinin is thus recommended as a nuclear stain for cervical cytology in the Papanicolaou procedure, both for image analysis and for visual microscopy.
研究了巴氏染色程序中使用的五种核染色剂的染色特性。用苏木精、酒精性硫堇溴化物、酒精性维多利亚蓝B、没食子蓝或硫堇福尔根试剂（硫堇-SO₂）作为核染色剂，采用改良的巴氏程序对酒精固定的宫颈涂片进行染色。所有玻片均使用相同的阴离子复染剂，并使用IBAS 2000图像分析仪测量细胞核和细胞质的光密度。酒精性硫堇产生的核染色最强，染色模式的重现性非常高。苏木精显示出染色强度的最高变异系数。苏木精和没食子蓝均产生一些非特异性细胞质染色。硫堇-SO₂可对DNA进行定量评估，但染色强度较低。金属络合染料染色会干扰随后用副品红福尔根试剂进行的染色。因此，推荐酒精性硫堇作为巴氏程序中宫颈细胞学的核染色剂，用于图像分析和目视显微镜检查。
