Morehead H W, Talmadge K W, O'Shannessy D J, Siebert C J
Bio-Rad Laboratories, Richmond, CA 94804.
J Chromatogr. 1991 Dec 20;587(2):171-6. doi: 10.1016/0021-9673(91)85153-7.
A rapid, simple assay for aldehydes generated by oxidation of saccharide units in glycoproteins, using dyes containing hydrazide functionalities, is described. The assay is used, in conjunction with tests of biological activity, to predict oxidation conditions that will result in a maximum of active protein coupled to a hydrazide chromatographic support. Glycoproteins are labeled with Lucifer Yellow CH or Texas Red Hydrazide, and the extent of labeling is determined. Using the assay, it is shown that the efficiency of coupling to Affi-Prep Hydrazide is proportional to oxidation.
本文描述了一种快速、简单的测定方法，该方法使用含有酰肼官能团的染料来检测糖蛋白中糖单元氧化产生的醛。该测定方法与生物活性测试相结合，用于预测氧化条件，以实现与酰肼色谱支持物偶联的活性蛋白产量最大化。糖蛋白用路西法黄CH或德克萨斯红酰肼进行标记，并测定标记程度。通过该测定方法表明，与Affi-Prep酰肼的偶联效率与氧化程度成正比。
