Spiegel S, Wilchek M, Fishman P H
Biochem Biophys Res Commun. 1983 May 16;112(3):872-7. doi: 10.1016/0006-291x(83)91698-4.
Lucifer yellow CH, a fluorescent hydrazide, reacted with cell surface glycoconjugates on murine thymocytes oxidized with NaIO4 or galactose oxidase. Surface labeling was monitored by fluorescence microscopy, spectrophotometry and by flow microfluorometry. No labeling was detected in unoxidized cells and fluorescence was reduced by neuraminidase and by trypsin. Lucifer yellow CH derivatives of gangliosides also were prepared and incorporated into the surface membranes of thymocytes.
荧光酰肼路西法黄CH与经高碘酸钠或半乳糖氧化酶氧化的小鼠胸腺细胞表面糖缀合物发生反应。通过荧光显微镜、分光光度法和流式微荧光法监测表面标记。在未氧化的细胞中未检测到标记，并且神经氨酸酶和胰蛋白酶可降低荧光。还制备了神经节苷脂的路西法黄CH衍生物，并将其掺入胸腺细胞的表面膜中。
