Riboni L, Luna F, Núñez-Durán H
Departamento de Ciencias Fisiológicas, Universidad Autónoma de Puebla, Mexico.
J Neurosci Methods. 1991 Jul;38(2-3):239-41. doi: 10.1016/0165-0270(91)90174-x.
We describe a staining technique for quick observation of the microanatomy of the vertebrate CNS. Successive soaking of the sections in tannic acid and ferric chloride produces a black colour in the gray matter. The procedure takes 5 min and is useful for identifying electrode penetrations, localization of lesions or teaching neuroanatomy.
我们描述了一种用于快速观察脊椎动物中枢神经系统微观解剖结构的染色技术。将切片依次浸泡在单宁酸和氯化铁中会使灰质呈现黑色。该过程耗时5分钟，对于识别电极穿刺、损伤定位或教授神经解剖学很有用。
