Mauger Chad A, Butler Ross N, Geier Mark S, Tooley Katie L, Howarth Gordon S
Centre for Paediatric and Adolescent Gastroenterology, Children, Youth and Women's Health Service, 72 King William Road, North Adelaide, South Australia, Australia.
Dig Dis Sci. 2007 Mar;52(3):612-9. doi: 10.1007/s10620-006-9464-y.
The sucrose breath test (SBT) was employed to noninvasively assess the efficacy of probiotics in 5-fluorouracil (5-FU)-induced intestinal mucositis. Dark Agouti rats were allocated to 5 groups (n = 10): 5-FU + L. fermentum BR 11, 5-FU + L. rhamnosus GG, 5-FU + B. lactis BB 12, 5-FU + skim milk (SM), and saline + SM. Probiotics were administered by oral gavage for 10 days. Mucositis was induced on day 7 by intraperitoneal injection of 5-FU (150 mg/kg) or vehicle (saline). Rats were sacrificed 72 h after 5-FU injection. The SBT measured breath 13CO2 (expressed as percentage cumulative dose at 90 min; %CD90) on days 0, 7, and 10. %CD90 was significantly lower in 5-FU-treated controls compared with that in saline-treated controls on day 10. 5-FU caused an 83% reduction in sucrase and a 510% increase in MPO activity. The SBT detected damage induced by 5-FU and is a simple, noninvasive indicator of small bowel injury. The probiotics assessed offered no protection from mucositis at the dose tested.
采用蔗糖呼气试验（SBT）对益生菌在5-氟尿嘧啶（5-FU）诱导的肠道黏膜炎中的疗效进行无创评估。将深色刺豚鼠分为5组（每组n = 10）：5-FU + 发酵乳杆菌BR 11组、5-FU + 鼠李糖乳杆菌GG组、5-FU + 乳酸双歧杆菌BB 12组、5-FU + 脱脂牛奶（SM）组和生理盐水 + SM组。通过口服灌胃给予益生菌，持续10天。在第7天通过腹腔注射5-FU（150 mg/kg）或赋形剂（生理盐水）诱导黏膜炎。在注射5-FU后72小时处死大鼠。在第0、7和10天，SBT测量呼出气体中的13CO2（以90分钟时的累积剂量百分比表示；%CD90）。在第10天，5-FU处理的对照组的%CD90显著低于生理盐水处理的对照组。5-FU导致蔗糖酶降低83%，髓过氧化物酶（MPO）活性增加510%。SBT检测到了5-FU诱导的损伤，是小肠损伤的一种简单、无创指标。在所测试的剂量下，评估的益生菌对黏膜炎没有保护作用。
