Del Rio J A, Soriano E, Ferrer I
Cell Biology Unit, Faculty of Biology, University of Barcelona, Spain.
Brain Res Dev Brain Res. 1991 Dec 17;64(1-2):205-11. doi: 10.1016/0165-3806(91)90227-a.
Immunocytochemical methods were used to investigate the developmental expression of substance P (SP) in mouse cerebral cortex. SP-like-immunoreactive cells were first detected at postnatal day 0 (P0), their numbers being notably increased by P2. Immunopositive cells were especially abundant in layer VIb and in the subjacent future white matter, although they were also present in layer V. Between P5 and P8 the number of SP-like-immunoreactive cells gradually decreased, being almost completely absent by P12. At these stages cells were only observed in the deepest cortical layers. From P16 onwards, the adult pattern of SP-like immunoreactivity emerged with a few immunopositive cells scattered throughout the cortical layers. The present data show a transitory population of SP-like-immunoreactive cells present in the mouse cerebral cortex during the first postnatal week. On the basis of close correlations of SP-like expression with the distribution or transitory populations and the timing of cell death in rodents, we propose that most of the SP-like-immunoreactive cells reported here would probably disappear by cell death.
采用免疫细胞化学方法研究小鼠大脑皮质中P物质（SP）的发育表达情况。出生后第0天（P0）首次检测到SP样免疫反应性细胞，到P2时其数量显著增加。免疫阳性细胞在VIb层和相邻的未来白质中特别丰富，尽管它们也存在于V层。在P5至P8之间，SP样免疫反应性细胞的数量逐渐减少，到P12时几乎完全消失。在这些阶段，仅在最深的皮质层观察到细胞。从P16开始，出现了成年期的SP样免疫反应模式，有一些免疫阳性细胞散布在整个皮质层。目前的数据显示，在出生后的第一周，小鼠大脑皮质中存在短暂的SP样免疫反应性细胞群体。基于SP样表达与啮齿动物中短暂群体的分布以及细胞死亡时间的密切相关性，我们提出此处报道的大多数SP样免疫反应性细胞可能会因细胞死亡而消失。
