Satoh J, Suzuki K
Department of Pathology, University of North Carolina, Chapel Hill 27599-7525.
Brain Res Dev Brain Res. 1990 Apr 1;53(1):1-5. doi: 10.1016/0165-3806(90)90119-j.
Using specific antiserum against tyrosine hydroxylase, the key enzyme in catecholamine synthesis, we found tyrosine hydroxylase immunoreactive neurons (TH-IN) in the cerebral cortex of Swiss Webster mice. They were found in the deeper layers of the cerebral isocortex in all the mice examined up to 60 days postnatal (P60). However, they appeared to be more numerous in early postnatal days. On P3 and P5, a few neurons with faintly stained perikarya were detected in the layer V and VI. The number and intensity of TH-IN increased gradually after P7, reaching to the maximum between P10 and P14 and then gradually decreased. Many of these neurons were multipolar but some were bipolar. Significance of TH-IN in the cortex and their apparent increase in immunoreactivity during the second postnatal week is not certain. Such an increase may be a transient increase in activity or phenotypic expression of tyrosine hydroxylase (TH) by young neurons in response to as yet unknown intrinsic environmental changes during this particular period of the brain development.
利用针对儿茶酚胺合成关键酶酪氨酸羟化酶的特异性抗血清，我们在瑞士韦伯斯特小鼠的大脑皮层中发现了酪氨酸羟化酶免疫反应性神经元（TH-IN）。在出生后60天（P60）之前检查的所有小鼠中，它们都存在于大脑新皮层的较深层。然而，它们在出生后的早期似乎数量更多。在P3和P5时，在V层和VI层检测到一些胞体染色较淡的神经元。TH-IN的数量和强度在P7后逐渐增加，在P10至P14之间达到最大值，然后逐渐下降。这些神经元中的许多是多极的，但也有一些是双极的。大脑皮层中TH-IN的意义以及它们在出生后第二周免疫反应性的明显增加尚不确定。这种增加可能是年轻神经元中酪氨酸羟化酶（TH）的活性或表型表达的短暂增加，以响应大脑发育这一特定时期尚不明确的内在环境变化。
