Guan Y H, Smulders J, Fisher D, Sutherland I A
Brunel Institute for Bioengineering, Brunel University West London, Uxbridge, Middlesex UB8 3PH, UK.
J Chromatogr A. 2007 Jun 1;1151(1-2):115-20. doi: 10.1016/j.chroma.2006.12.097. Epub 2007 Jan 8.
Retention properties of polyethylene glycol-phosphate aqueous two-phase systems in a spiral coil (5 mm I.D.) on Type-J synchronous counter-current chromatographic devices have been compared for the elution mode where the lower phase is the mobile phase and flows from the inside head terminal. This was achieved with the aid of digital imaging under stroboscopic illumination, an image analysis and measurement of the displaced volume of the stationary phase. For the spiral coil, high and stable stationary phase retention at mobile phase flow rates up to 64 ml/min has been obtained. Wave-like disturbance of the interface near the proximal point was observed and analyses have been made for possible use in protein separation.
在内径为5毫米的螺旋盘管中，对聚乙二醇 - 磷酸盐双水相系统在J型同步逆流色谱装置上的保留特性进行了比较，洗脱模式为下相作为流动相并从内部头部终端流出。这借助频闪照明下的数字成像、图像分析以及固定相位移体积的测量得以实现。对于螺旋盘管，在高达64毫升/分钟的流动相流速下获得了高且稳定的固定相保留率。观察到近端附近界面的波状扰动，并对其在蛋白质分离中的可能应用进行了分析。
