Ito Yoichiro, Qi Lin, Powell Jimmie, Sharpnack Frank, Metger Howard, Yost James, Cao Xue-Li, Dong Yin-Mao, Huo Liang-Sheng, Zhu Xiao-Ping, Li Ting
Center for Biochemistry and Biophysics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-8014, USA.
J Chromatogr A. 2007 Jun 1;1151(1-2):108-14. doi: 10.1016/j.chroma.2006.11.078. Epub 2007 Feb 21.
A novel spiral disk is designed by placing barricades at 6 mm intervals in the middle of the spiral channel to divide the channel into multiple sections. Glass beads are placed in every other section so that the planetary motion produces repetitive mixing and settling of polymer phase systems. Performance of this mixer-settler spiral disk assembly was examined for separation of lysozyme and myoglobin with a polymer phase system. The best results were obtained with a spiral disk equipped with barricades with openings ranging from 1.2 to 0.4 mm on each side at a high revolution speed up to 1200 rpm.
一种新型螺旋盘是通过在螺旋通道中间每隔6毫米设置障碍物来设计的，这些障碍物将通道分成多个部分。玻璃珠放置在每隔一个的部分中，以便行星运动使聚合物相体系产生重复的混合和沉降。使用聚合物相体系对这种混合沉降螺旋盘组件进行了溶菌酶和肌红蛋白分离的性能测试。在转速高达1200转/分钟的情况下，使用两侧开口范围为1.2至0.4毫米的带障碍物螺旋盘可获得最佳结果。
