Spanevello Roselia M, Mazzanti Cinthia M, Maldonado Paula A, Zanin Rafael, Morsch André, Hannel Laura, Mazzanti Alexandre, Festugatto Rafael, Graça Dominguita, Schmatz Roberta, Loro Vânia Lúcia, Schetinger Maria Rosa C, Morsch Vera M
Pós Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
Life Sci. 2007 Feb 27;80(12):1109-14. doi: 10.1016/j.lfs.2006.11.049. Epub 2006 Dec 8.
The activities of the enzymes NTPDase (EC 3.6.1.5, apyrase, CD39) and 5'-nucleotidase (EC 3.1.3.5, CD73) were analyzed in platelets from rats submitted to demyelination by ethidium bromide (EB) and treated with interferon beta (IFN-beta). The following groups were studied: I - control (saline), II - (saline and IFN-beta), III - (EB) and IV - (EB and IFN-beta). After 7, 15 and 30 days, the animals (n=7) were sacrificed and the platelets were separated by the method of Lunkes et al. [Lunkes, G., Lunkes D., Morsch, V., Mazzanti, C., Morsch, A., Miron, V., Schetinger, M.R.C., 2004. NTPDase and 5'-nucleotidase in rats alloxan- induced diabetes. Diabetes Research and Clinical Practice 65, 1-6]. NTPDase activity for ATP and ADP substrates was significantly lower in groups II and III after seven days, when compared to control (p<0.001). At fifteen days, ATP hydrolysis was significantly lower in group III and IV and higher in group II (p<0.001), while there was an activation of ADP hydrolysis in group II (p<0.001), when compared with the control. 5'-nucleotidase activity was significantly higher in group IV (p<0.001) after seven days, and lower in the groups III and IV (p<0.001) after fifteen days in relation to the control. No significant differences were observed in NTPDase and 5'-nucleotidase activities after thirty days. In conclusion, our study demonstrated that the hydrolysis of adenine nucleotides is modified in platelets of rats demyelinated and treated with IFN-beta.
对用溴化乙锭（EB）诱导脱髓鞘并接受干扰素β（IFN-β）治疗的大鼠血小板中的NTPD酶（EC 3.6.1.5，腺苷三磷酸双磷酸酶，CD39）和5'-核苷酸酶（EC 3.1.3.5，CD73）的活性进行了分析。研究了以下几组：I组 - 对照组（生理盐水），II组 - （生理盐水和IFN-β），III组 - （EB）和IV组 - （EB和IFN-β）。7天、15天和30天后，处死动物（n = 7），并采用Lunkes等人的方法分离血小板[Lunkes, G., Lunkes D., Morsch, V., Mazzanti, C., Morsch, A., Miron, V., Schetinger, M.R.C., 2004. 大鼠四氧嘧啶诱导糖尿病中的NTPD酶和5'-核苷酸酶。糖尿病研究与临床实践65, 1 - 6]。与对照组相比，7天后II组和III组中针对ATP和ADP底物的NTPD酶活性显著降低（p < 0.001）。15天时，III组和IV组中ATP水解显著降低，II组中ATP水解显著升高（p < 0.001），而与对照组相比，II组中ADP水解有激活（p < 0.001）。7天后IV组中5'-核苷酸酶活性显著升高（p < 0.001），15天后III组和IV组中5'-核苷酸酶活性相对于对照组显著降低（p < 0.001）。30天后，NTPD酶和5'-核苷酸酶活性未观察到显著差异。总之，我们的研究表明，脱髓鞘并接受IFN-β治疗的大鼠血小板中腺嘌呤核苷酸的水解发生了改变。
