Department of Biochemistry, Institute of Basic Sciences for Health, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
J Neurol. 2010 Jan;257(1):24-30. doi: 10.1007/s00415-009-5258-4. Epub 2009 Jul 24.
Multiple sclerosis (MS) is the most common chronic disabling neurological disease in young adults. Alterations in platelet function have been observed in MS; however, the mechanism and the relevance of this blood cell disorder with regard to MS pathogenesis are not yet understood. The aim of this study was to evaluate activities of ectonucleoside thiphosphate diphosphohydrolase (NTPDase, CD39), ectonucleotide pyrophosphatase/phosphodiesterase (E-NPP), 5'-nucleotidase and adenosine deaminase (ADA) in platelets from patients with the relapsing-remitting form of MS (RRMS), as well as to analyze platelet aggregation and expression of NTPDase. The results obtained show that NTPDase, 5'-nucleotidase, E-NPP and ADA activities were decreased in platelets of RRMS patients when compared with the control group (p < 0.05). In addition, NTPDase expression in platelets was also decreased in these patients (p < 0.05); however, no differences were observed in platelet aggregation between RRMS patients and the control group. Our results suggest that the alterations in NTPDase, E-NPP, 5'-nucleotidase and ADA may have contributed to the alterations in platelet function in MS by altering the levels of nucleotides and nucleosides in the circulation.
多发性硬化症（MS）是年轻人中最常见的慢性致残性神经疾病。已经观察到血小板功能的改变在 MS 中;然而，这种血细胞紊乱与 MS 发病机制的关系及其机制尚不清楚。本研究旨在评估复发性缓解型多发性硬化症（RRMS）患者血小板中核苷酸三磷酸二磷酸水解酶（NTPDase，CD39）、核苷酸焦磷酸酶/磷酸二酯酶（E-NPP）、5'-核苷酸酶和腺苷脱氨酶（ADA）的活性，并分析血小板聚集和 NTPDase 的表达。研究结果表明，与对照组相比，RRMS 患者的血小板中 NTPDase、5'-核苷酸酶、E-NPP 和 ADA 的活性降低（p<0.05）。此外，这些患者的血小板中 NTPDase 的表达也降低（p<0.05）;然而，RRMS 患者和对照组之间的血小板聚集没有差异。我们的研究结果表明，NTPDase、E-NPP、5'-核苷酸酶和 ADA 的改变可能通过改变循环核苷酸和核苷的水平，导致 MS 中血小板功能的改变。
