Peng Baowei, Zhao Yonggang, Xu Lingyun, Xu Yuhong
School of Pharmacy, Shanghai JiaoTong University, 1954 Hua Shan Rd., Shanghai 200030, PR China.
Vaccine. 2007 Mar 1;25(11):2064-73. doi: 10.1016/j.vaccine.2006.11.042. Epub 2006 Dec 8.
Electroporation can improve intramuscular DNA vaccination efficacy but the exact antigen presentation mechanism remains unclear. We reported here that a similar immuno-potentiation effect was also observed by stimulating the skeletal muscles with electric pulses (EP) a few days prior to DNA inoculation (EP + n days + DNA). The application of EP by itself activated proinflammatory chemokine genes and stress genes. It also triggered an influx of inflammatory monocytes/macrophages (MPs). After DNA inoculation, the plasmids were seen taken up by these inflammatory MPs, which migrated to the draining LNs subsequently. The antibody responses results were fast and strong. Furthermore, MPs isolated from the draining LNs of EP + n days + DNA treated mice were capable of stimulating Ag specific CD4+ T cell proliferation in vitro. Based on these observations, we proposed that the local inflammation resulted from EP treatment played an important role in facilitating antigen presentation of the DNA vaccines.
电穿孔可提高肌内DNA疫苗的效力，但确切的抗原呈递机制仍不清楚。我们在此报告，在DNA接种前几天用电脉冲（EP）刺激骨骼肌（EP + n天+ DNA）也观察到了类似的免疫增强作用。单独应用EP可激活促炎趋化因子基因和应激基因。它还引发了炎性单核细胞/巨噬细胞（MPs）的流入。DNA接种后，可见这些炎性MPs摄取质粒，随后迁移至引流淋巴结。抗体反应结果快速且强烈。此外，从EP + n天+ DNA处理小鼠的引流淋巴结中分离出的MPs能够在体外刺激抗原特异性CD4 + T细胞增殖。基于这些观察结果，我们提出EP治疗引起的局部炎症在促进DNA疫苗的抗原呈递中起重要作用。
