Fei Yurong, Zhang Min, Li Ming, Huang Yuying, He Wei, Ding Wenjun, Yang Jianhong
Department of Biology, Graduate University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, PR China.
Micron. 2007;38(6):637-42. doi: 10.1016/j.micron.2006.09.003. Epub 2006 Oct 12.
Diabetes mellitus affects bone metabolism and leads to osteopenia and osteoporosis, but its pathogenic mechanism remains unknown. To address this problem, mineral element of bone was analyzed in experimental diabetic osteoporosis model. Male Wistar rats were randomly divided into streptozotocin (STZ)-induced diabetic group (n=5) and control group (n=5). The experiment lasted 68 days and at the end of the experiment, femoral bone mineral density (BMD) was measured by dual-energy X-ray absorptiometry and element content in femur of animals was determined by synchrotron radiation X-ray fluorescence (SRXRF) microprobe analysis technique. Results showed that femoral BMD in diabetic group was significantly lower than that in control (P<0.01). Relative mineral content of calcium (Ca), phosphorus (P) and zinc (Zn) in diabetic femurs decreased significantly compared to controls. And strontium (Sr) in diabetics reduced 11% (P=0.09). Relative content of sulfur (S) in average was statistically higher (P<0.01) in diabetics than that in controls. But no obvious difference was observed in relative content of chromium (Cr), iron (Fe), copper (Cu), and lead (Pb) between the two groups. Statistical analysis revealed that Ca correlated positively with P (R=0.85 and P<0.001), with Sr (R=0.38 and P<0.05) and with Zn (R=0.37 and P<0.05). Whereas, Zn correlated negatively with S (R=-0.40 and P<0.05). Our results reveal that loss of minerals accounts for the BMD reduction in diabetics.
糖尿病会影响骨代谢，导致骨质减少和骨质疏松，但其致病机制尚不清楚。为解决这一问题，在实验性糖尿病骨质疏松模型中对骨矿物质元素进行了分析。将雄性Wistar大鼠随机分为链脲佐菌素（STZ）诱导的糖尿病组（n = 5）和对照组（n = 5）。实验持续68天，实验结束时，采用双能X线吸收法测量股骨骨密度（BMD），并通过同步辐射X射线荧光（SRXRF）微探针分析技术测定动物股骨中的元素含量。结果显示，糖尿病组的股骨BMD显著低于对照组（P<0.01）。与对照组相比，糖尿病大鼠股骨中钙（Ca）、磷（P）和锌（Zn）的相对矿物质含量显著降低。糖尿病大鼠体内的锶（Sr）减少了11%（P = 0.09）。糖尿病大鼠体内硫（S）的平均相对含量在统计学上高于对照组（P<0.01）。但两组之间铬（Cr）、铁（Fe）、铜（Cu）和铅（Pb）的相对含量未观察到明显差异。统计分析表明，Ca与P呈正相关（R = 0.85，P<0.001），与Sr呈正相关（R = 0.38，P<0.05），与Zn呈正相关（R = 0.37，P<0.05）。而Zn与S呈负相关（R = -0.40，P<0.05）。我们的结果表明，矿物质流失是糖尿病患者BMD降低的原因。
