College of Life Sciences, Graduate University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, People's Republic of China.
J Biomed Mater Res A. 2011 Dec 1;99(3):386-94. doi: 10.1002/jbm.a.33201. Epub 2011 Aug 23.
Pure magnesium (Mg) was implanted intramedullary into the femur of streptozotocin (STZ)-induced diabetic rats to investigate its effect on bone growth after 6 weeks degradation. The experimental results showed that the femoral BMD in diabetic rats was significantly lower than that in controls (p < 0.01) but restored notably by Mg implantation. The contents of calcium (Ca), phosphorus (P), Mg, zinc (Zn), potassium (K), strontium (Sr), and sulfur (S) in bone of diabetic group were significantly lower than those in controls but remarkably increased with implantation of Mg. The residual weight calculation showed that 29.41% of Mg was degraded in vivo. The energy dispersive X-ray spectroscopy (EDS) analysis showed that the reaction layer on the surface of the Mg implant mainly consisted of C, Ca, O, P, and Mg. Besides, serum Mg level was significantly decreased in diabetic group compared with the control group but increased by Mg treatment. Also, there were no significant differences in body weight and blood glucose, as well as blood glycosylated hemoglobin (HbAIc%), serum Ca, alanine aminitransperase (ALT), aspartate aminotransferase (AST), uric acid (UA), nonesterified fatty acid (NEFA), cholinesterase (CHE), and creatinine (CR) levels between diabetic and Mg-implanted rats. The study indicated that Mg implant had no obvious toxicity in STZ-induced diabetic rats and may act as a potential agent to treat osteoporosis.
纯镁（Mg）被植入链脲佐菌素（STZ）诱导的糖尿病大鼠的骨髓腔中，以研究其在 6 周降解后对骨生长的影响。实验结果表明，糖尿病大鼠的股骨骨密度明显低于对照组（p<0.01），但植入 Mg 后明显恢复。糖尿病组骨中钙（Ca）、磷（P）、镁（Mg）、锌（Zn）、钾（K）、锶（Sr）和硫（S）的含量明显低于对照组，但植入 Mg 后显著增加。残留重量计算表明，体内 29.41%的 Mg 发生了降解。能谱分析（EDS）表明，Mg 植入物表面的反应层主要由 C、Ca、O、P 和 Mg 组成。此外，与对照组相比，糖尿病组血清 Mg 水平显著降低，但经 Mg 处理后升高。糖尿病和 Mg 植入大鼠的体重、血糖以及血糖糖化血红蛋白（HbAIc%）、血清 Ca、丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、尿酸（UA）、非酯化脂肪酸（NEFA）、胆碱酯酶（CHE）和肌酐（CR）水平均无显著差异。研究表明，Mg 植入物在 STZ 诱导的糖尿病大鼠中没有明显的毒性，可能是治疗骨质疏松症的潜在药物。
