Wang Shumin, Zhou Lei, He Wenying, Hu Zhide
Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
J Pharm Biomed Anal. 2007 Mar 12;43(4):1557-61. doi: 10.1016/j.jpba.2006.11.021. Epub 2007 Jan 19.
A novel electokinetic capillary chromatography method, reverse sodium dodecyl sulfate (SDS) micelles as pseudo-stationary phase, was developed for separation and detection of alpinetin and cardamonin. In this work, reverse micelles (RMs) have been firstly introduced into background electrolyte for electrophoresis separation. The optimum reverse SDS micelle system was formed with n-butyl chloride as continuous phase, SDS (20.9%, w/v) as the surfactant, W(0) (13.0, water-surfactant molar ratio), 18.0% (v/v) 1-butanol as the co-surfactant, 8.0% (v/v) acetonitrile (ACN), 1.5% (v/v) heptane, and a 60 mol L(-1) tris-(hydroxymethyl)aminomethane (Tris) buffer, as dispersed phase. Linear relationships (correlation coefficients: 0.9961 for cardamonin and 0.9991 for alpinetin) between the peak areas and concentration of the two compounds were obtained (5.0-350.0 microg mL(-1) for cardamonin and 1.25-350.0 microg mL(-1) for alpinetin). The detection limits (S/N=3) for cardamonin and alpinetin were 0.19 and 0.14 microg mL(-1), respectively. The method was successfully applied for the quantification of alpinetin and cardamonin in Alpinia katsumadai Hayata and kuaiwei tablet with satisfactory recoveries in the range of 95.9-100.2%.
建立了一种新型电动毛细管色谱方法，以反向十二烷基硫酸钠（SDS）胶束作为假固定相，用于分离和检测山姜素和小豆蔻明。在本研究中，首次将反向胶束（RMs）引入背景电解质中进行电泳分离。最佳反向SDS胶束体系以正丁基氯为连续相，SDS（20.9%，w/v）为表面活性剂，W(0)（13.0，水-表面活性剂摩尔比），18.0%（v/v）正丁醇为共表面活性剂，8.0%（v/v）乙腈（ACN），1.5%（v/v）庚烷，以及60 mol L(-1)三（羟甲基）氨基甲烷（Tris）缓冲液作为分散相。获得了两种化合物的峰面积与浓度之间的线性关系（相关系数：小豆蔻明为0.9961，山姜素为0.9991）（小豆蔻明为5.0 - 350.0 μg mL(-1)，山姜素为1.25 - 350.0 μg mL(-1)）。小豆蔻明和山姜素的检测限（S/N = 3）分别为0.19和0.14 μg mL(-1)。该方法成功应用于草豆蔻和开胃片中山姜素和小豆蔻明的定量分析，回收率在95.9 - 100.2%范围内，结果令人满意。
