流动注射-胶束电动色谱法分离测定草豆蔻中山姜素和小豆蔻明
Separation and determination of alpinetin and cardamonin in Alpinia katsumadai Hayata by flow injection-micellar electrokinetic chromatography.
作者信息
Liu Lihong, Chen Xingguo, Hu Zhide
机构信息
Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
出版信息
Talanta. 2007 Jan 15;71(1):155-9. doi: 10.1016/j.talanta.2006.03.032. Epub 2006 May 5.
A simple, rapid, and accurate method for the separation and determination of alpinetin and cardamonin in Alpinia katsumadai Hayata was developed by combination of flow injection (FI)-micellar electrokinetic chromatography (MEKC) for the first time. The analysis was carried out using an unmodified fused-silica capillary (50microm i.d.; total length 13.6cm; effective length 10.3cm) and direct ultraviolet (UV) detection at 214nm. The sample throughput was 11-24 samples per hour using the background electrolyte (BGE) containing 4mM sodium borate-8mM NaH(2)PO(4) (pH 8.1)-8mM sodium dodecyl sulfate (SDS)-19% (v/v) ethanol. The repeatabilities (n=4) reached relative standard deviation values (R.S.D.) of 3.0% and 2.5% for the peak areas and 2.5% and 3.1% for peak heights of alpinetin and cardamonin, respectively. Regression equations revealed linear relationships (r(2): 0.9993-0.9994) between the peak area of each analyte and the concentration. Recoveries were in the range 90-92% and 99-105% for alpinetin and cardamonin, respectively.
首次通过流动注射（FI）-胶束电动色谱（MEKC）联用技术，开发了一种简单、快速且准确的方法，用于分离和测定草豆蔻中红豆蔻查耳酮和小豆蔻明。分析采用未修饰的熔融石英毛细管（内径50μm；总长13.6cm；有效长度10.3cm），并在214nm处进行直接紫外（UV）检测。使用含有4mM硼酸钠-8mM NaH₂PO₄（pH 8.1）-8mM十二烷基硫酸钠（SDS）-19%（v/v）乙醇的背景电解质（BGE）时，样品通量为每小时11-24个样品。重复性（n = 4）方面，红豆蔻查耳酮和小豆蔻明的峰面积相对标准偏差（R.S.D.）分别为3.0%和2.5%，峰高相对标准偏差分别为2.5%和3.1%。回归方程显示各分析物的峰面积与浓度之间呈线性关系（r²：0.9993 - 0.9994）。红豆蔻查耳酮和小豆蔻明的回收率分别在90 - 92%和99 - 105%范围内。
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