Deng Jing, Chen Guo-Yuan, Ji Jia-Jia, Tan Hao, Wang Song, Hu Feng-Xian, Chai Lian-Hua, Liu Si-Hai, Liu Wei-Dong
Department of Labor Health & Environment Health, Tongji Medical College of Huazhong University of Science & Technology, Wuhan 430030, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2006 Dec;24(12):730-3.
To study apoptosis and gene FasL expression induced by carbon disulfide in sertoli cells of male rats.
Sertoli cells were exposed to different concentrations of CS(2) (0, 0.36, 0.72, 1.44 micromol/ml) for 24 hours. Survival rate, apoptosis rate, expression level of gene FasL were measured using MTT, FCM, and RT-PCR methods respectively.
Sertoli cell survival rate decreased as the concentration of CS(2) increased. The survival rate (73.34% +/- 1.39%) was significantly lower than the control group (99.98% +/- 5.48%) when the concentration of CS(2) > or = 1.44 micromol/ml (P < 0.05). Apoptosis rate increased as the CS(2) concentration increased. Apoptosis rate (7.93% +/- 0.43%) was significantly higher when the concentration of CS(2) > or = 1.44 micromol/ml (P < 0.05). Expression level of the FasL significantly increased as the concentrations of CS(2) (P < 0.05).
CS(2) is cytotoxic to sertoli cells. It could cause apoptosis of sertoli cells.
