Mjellem-Joly N, Lund A, Berge O G, Hole K
Departement of Physiology, University of Bergen, Norway.
Neurosci Lett. 1991 Nov 25;133(1):121-4. doi: 10.1016/0304-3940(91)90072-2.
The functional interaction in the spinal cord between substance P and excitatory amino acid agonists was investigated. Behavioural responses were scored in mice after intrathecal administration of excitatory amino acid agonists and substance P, given separately or in combination. A strong potentiation of the effect was seen when substance P was coadministered with N-methyl-D-aspartate (NMDA), alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) or kainic acid (KA). The potentiation was blocked by the corresponding antagonists: the selective NMDA-receptor antagonist (+/-)-3- (2-carboxypiperazin-4-yl)propyl-1-phosphonic acid (CPP), the non-NMDA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and the substance P analog, [D-Arg1,D-Trp7,9,Leu11]-substance P (Spantide). These findings indicate a functional interaction between substance P and glutamate in the dorsal horn of the spinal cord, compatible with the hypothesis that corelease of substance P and glutamate from primary afferent neurons may enhance nociception.
研究了P物质与兴奋性氨基酸激动剂在脊髓中的功能相互作用。分别或联合鞘内注射兴奋性氨基酸激动剂和P物质后，对小鼠的行为反应进行评分。当P物质与N-甲基-D-天冬氨酸（NMDA）、α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）或 kainic 酸（KA）共同给药时，可观察到效应的强烈增强。这种增强作用被相应的拮抗剂阻断：选择性NMDA受体拮抗剂（±）-3-（2-羧基哌嗪-4-基）丙基-1-膦酸（CPP）、非NMDA受体拮抗剂6-氰基-7-硝基喹喔啉-2,3-二酮（CNQX）和P物质类似物[D-Arg1,D-Trp7,9,Leu11]-P物质（Spantide）。这些发现表明P物质与脊髓背角中的谷氨酸之间存在功能相互作用，这与初级传入神经元同时释放P物质和谷氨酸可能增强伤害感受的假说相符。
