[甲型肝炎病毒衣壳蛋白片段寡肽的合成及免疫化学特性]

[Synthesis and immunochemical properties of oligopeptides--fragments of capsid proteins of the hepatitis A virus].

作者信息

Chikin L D, Kozhich A T, Ivanov V S, Ivanov V T, Nastashenko T A, Kusov Iu Iu, Balaian M S

出版信息

Bioorg Khim. 1991 Jul;17(7):964-72.

Abstract

The hepatitis A virus (HAV) capsid protein VP1, VP2 and VP3 are exposed at the virion surface and should therefore contain antigenic determinants. Algorithms for hydrophilicity, antigenicity and flexibility were used to predict probable antigenic sites. Synthesis of 7- to 23-membered overlapping peptides from seven sites, viz., 1-11, 1-17, 2-33, 11-25, 73-82, 76-86, 98-109, 98-112, 102-107, 102-108, 108-127, 113-123, 118-140, 276-298 from VP1, 42-62 from VP2, 76-85 from VP3, and 1-23 from VP4, was performed by various solid-phase methods. Free peptides and their conjugates with different carriers were used for immunization and study of antigenicity. The peptides did not interact with antibodies to the hepatitis A virus, whereas their conjugates did not induce the formation of anti-HAV-antibodies.

摘要

甲型肝炎病毒（HAV）的衣壳蛋白VP1、VP2和VP3暴露于病毒粒子表面，因此应含有抗原决定簇。利用亲水性、抗原性和柔韧性算法预测可能的抗原位点。通过各种固相方法合成了来自七个位点的7至23元重叠肽，这些位点分别是：VP1的1-11、1-17、2-33、11-25、73-82、76-86、98-109、98-112、102-107、102-108、108-127、113-123、118-140、276-298，VP2的42-62，VP3的76-85，以及VP4的1-23。游离肽及其与不同载体的缀合物用于免疫和抗原性研究。这些肽不与甲型肝炎病毒抗体相互作用，而它们的缀合物也不诱导抗HAV抗体的形成。