Semiletov Iu A, Karpova V A, Khudiakov Iu E, Pavliuchenkova R P, Viazov S O, Smirnov V D, Prokuronova E I, Zheltukhina G A, Evstigneeva R P
Bioorg Khim. 1992 Feb;18(2):252-62.
Hepatitis delta virus (HDV), a recently discovered infectious agent, participates in severe, often lethal forms of acute and chronic hepatitis and liver cirrhosis. Based on theoretical analysis of secondary structure, hydrophilicity and acrophilicity data, several regions of HDV antigen, presumably containing B-epitopes, have been revealed and the corresponding peptides have been synthesized by the solid phase method. All the peptides obtained reacted with the respective antipeptide rabbit sera. The peptides and their conjugates with BSA or KLH were used for ELISA with individual and pooled anti-HD-positive sera from patients with chronic delta hepatitis. The high antigenicity of the peptide 65-80 shows that one of the antigenically active regions of HDAg is situated between these amino acid residues and that the peptide may be used for detection of anti-HD antibodies in patients blood sera.
丁型肝炎病毒（HDV）是一种最近发现的感染因子，可引发严重的、往往致命的急慢性肝炎及肝硬化。基于对二级结构、亲水性和嗜碱性数据的理论分析，已揭示出HDV抗原的几个区域可能含有B表位，并通过固相法合成了相应的肽段。所有获得的肽段均与各自的抗肽兔血清发生反应。这些肽段及其与牛血清白蛋白（BSA）或钥孔戚血蓝蛋白（KLH）的缀合物用于慢性丁型肝炎患者个体和混合抗HD阳性血清的酶联免疫吸附测定（ELISA）。肽段65 - 80的高抗原性表明，丁型肝炎抗原（HDAg）的一个抗原活性区域位于这些氨基酸残基之间，该肽段可用于检测患者血清中的抗HD抗体。
