甲型肝炎病毒VP2蛋白中连续B表位的定位

Localization of a sequential B-epitope in the VP2 protein of hepatitis A virus.

作者信息

Kulik L N, Ivanov V S, Tchikin L D, Ostrovsky A G, Ivanov V T

机构信息

Institute of Bioorganic Chemistry, Byelorussian Academy of Sciences, Minsk.

出版信息

FEBS Lett. 1995 Jun 19;367(1):85-8. doi: 10.1016/0014-5793(95)00520-j.

DOI:10.1016/0014-5793(95)00520-j

PMID:7541374

Abstract

A set of synthetic peptides derived from the capsid protein of hepatitis A virus was used to search for B-epitopes. Peptides from the 115-139 region of the VP1 protein, from the 69-99 region of the VP2 protein and peptide 137-150 from the VP3 protein were found to react with monoclonal and polyclonal anti-HAV antibodies. MAPs based on 64-80 and 66-80 fragments of VP3 were reactive as well. Peptides, their conjugates with protein carriers and MAPs were used for antipeptide antibody production. Only free peptide 69-99 from the VP2 protein caused formation of HAV binding antibodies.

摘要

一组源自甲型肝炎病毒衣壳蛋白的合成肽被用于搜索B细胞表位。发现来自VP1蛋白115 - 139区域的肽、来自VP2蛋白69 - 99区域的肽以及来自VP3蛋白的肽137 - 150能与单克隆和多克隆抗甲型肝炎病毒抗体发生反应。基于VP3蛋白64 - 80和66 - 80片段的多抗原肽也具有反应活性。肽、它们与蛋白质载体的缀合物以及多抗原肽被用于抗肽抗体的产生。只有来自VP2蛋白的游离肽69 - 99能引起甲型肝炎病毒结合抗体的形成。