Gan B K, Kondyurin A, Bilek M M M
Applied and Plasma Physics, School of Physics (A28), The University of Sydney, New South Wales 2006, Australia.
Langmuir. 2007 Feb 27;23(5):2741-6. doi: 10.1021/la062722v. Epub 2007 Jan 23.
Surface attachment of the enzyme horseradish peroxidase (HRP) was studied on untreated and ion beam implanted polystyrene (PS) films. The PS films of 100 nm thickness on a silicon wafer were treated using the plasma immersion ion implantation (PIII) technique, with argon ions of energy 20 keV and fluences of up to 2 x 10(16) ions/cm2. Differential transmittance Fourier transform infrared (FTIR) spectra confirmed the presence of proteins on the PS surfaces by detection of the amide A, I, and II protein vibrations. Spectroscopic ellipsometry over the UV-vis spectral region provided the optical constants and thickness of the protein layer, while tapping mode atomic force microscopy (AFM) was used to image the protein distribution on the surface. The combination of AFM, ellipsometry, and FTIR analysis showed that, on the untreated PS surface, HRP formed islands 8 nm in height and 30 nm in lateral size, covering approximately 27% of the PS surface. After PIII modification of the PS surface, the protein covered 100% of the surface area.
研究了辣根过氧化物酶（HRP）在未处理和离子束注入的聚苯乙烯（PS）薄膜上的表面附着情况。使用等离子体浸没离子注入（PIII）技术对硅片上厚度为100 nm的PS薄膜进行处理，氩离子能量为20 keV，通量高达2×10¹⁶离子/cm²。差分透射傅里叶变换红外（FTIR）光谱通过检测酰胺A、I和II蛋白质振动证实了PS表面存在蛋白质。紫外-可见光谱区域的光谱椭偏仪提供了蛋白质层的光学常数和厚度，而轻敲模式原子力显微镜（AFM）用于成像表面蛋白质分布。AFM、椭偏仪和FTIR分析相结合表明，在未处理的PS表面，HRP形成了高度为8 nm、横向尺寸为30 nm的岛状结构，覆盖了约27%的PS表面。PS表面经过PIII改性后，蛋白质覆盖了100%的表面积。
