Hui Elliot E, Bhatia Sangeeta N
Department of Bioengineering, University of California, San Diego, La Jolla, California, USA.
Langmuir. 2007 Apr 10;23(8):4103-7. doi: 10.1021/la0630559. Epub 2007 Jan 23.
The complexity of micropatterned cell constructs has been limited by difficulties in patterning more than two surface components on a culture substrate. Photolithography using multiple aligned masks is well established for generalized multicomponent patterning, but is often too harsh for biomolecules. We report a two-mask photolithographic process that is tuned to preserve bioactivity in patterns composed of covalently coupled poly(ethylene glycol) (PEG), adsorbed extracellular matrix protein (e.g., collagen I), and adsorbed serum proteins (e.g., vitronectin). Thereby, we pattern two cell types-primary hepatocytes and 3T3 fibroblasts-demonstrating control over contact and spacing (20-200 microm) between the two cell types for over one week. This method is applicable to the study of intercellular communication in cell biology and tissue engineering.
微图案化细胞构建体的复杂性受到在培养底物上对两种以上表面成分进行图案化时所遇到困难的限制。使用多个对齐掩膜的光刻技术已被广泛用于广义的多组分图案化，但对于生物分子来说往往过于苛刻。我们报告了一种双掩膜光刻工艺，该工艺经过调整，可在由共价偶联的聚乙二醇（PEG）、吸附的细胞外基质蛋白（如I型胶原蛋白）和吸附的血清蛋白（如玻连蛋白）组成的图案中保留生物活性。由此，我们对两种细胞类型——原代肝细胞和3T3成纤维细胞进行了图案化，证明了在一周多的时间内对两种细胞类型之间的接触和间距（20 - 200微米）具有控制能力。该方法适用于细胞生物学和组织工程中细胞间通讯的研究。
