Lauer L, Ingebrandt S, Scholl M, Offenhäusser A
Max Planck Institute for Polymer Research, Mainz, Germany.
IEEE Trans Biomed Eng. 2001 Jul;48(7):838-42. doi: 10.1109/10.930910.
Microcontact printing (muCP) of extracellular matrix proteins is a fascinating approach to control cell positioning and outgrowth, which is essential in the development of applications ranging from cellular biosensors to tissue engineering. Microelectronic devices can be used to detect the activity from a large number of recording sites over the long term. However, signals from cells can only be recorded at small sensitive spots. In this paper, we present an innovative setup to perform aligned muCP of extracellular matrix proteins on microelectronic devices in order to guide the growth of electrogenic cells specifically to these sensitive spots. Our system is based on the combination of a fine-placer with redesigned micro stamps having a rigid glass cylinder as backbone for attachment in the alignment tool. Alignment is performed moving the device with an optical table under microscopic control of the superimposed images from stamp and device surface. After successful alignment, the stamp is brought into contact with the device surface by means of a high-precision lever. With our setup, we were able to pattern up to 40 devices per hour. A lateral alignment accuracy of < 2microm has been achieved. Aligned neuronal growth on patterned devices was demonstrated with dissociated hippocampal neurons.
细胞外基质蛋白的微接触印刷（μCP）是一种控制细胞定位和生长的迷人方法，这在从细胞生物传感器到组织工程等各种应用的开发中至关重要。微电子设备可用于长期检测来自大量记录位点的活动。然而，来自细胞的信号只能在小的敏感点上记录。在本文中，我们提出了一种创新装置，用于在微电子设备上进行细胞外基质蛋白的对齐微接触印刷，以便将电genic细胞的生长特异性地引导到这些敏感点。我们的系统基于精细放置器与重新设计的微印章的组合，该微印章具有刚性玻璃圆柱体作为在对齐工具中附着的主干。通过在光学平台上移动设备并在来自印章和设备表面的叠加图像的显微镜控制下进行对齐。成功对齐后，通过高精度杠杆使印章与设备表面接触。使用我们的装置，我们能够每小时对多达40个设备进行图案化。实现了<2微米的横向对齐精度。用解离的海马神经元证明了在图案化设备上的对齐神经元生长。
