Nakanishi Mio, Hamazaki Tatsuo S, Komazaki Shinji, Okochi Hitoshi, Asashima Makoto
Department of Life Science (Biology), Graduate School of Arts & Science, University of Tokyo, Meguro, Tokyo 153-8902, Japan.
Differentiation. 2007 Jan;75(1):1-11. doi: 10.1111/j.1432-0436.2006.00109.x.
The in vitro formation of organs and/or tissues is a major goal for regenerative medicine that would also provide a powerful tool for analyzing both the mechanisms of development and disease processes for each target organ. Here, we present a method whereby pancreatic tissues can be formed in vitro from mouse embryonic stem (ES) cells. Embryoid body-like spheres (EBSs) induced from ES cell colonies were treated with retinoic acid (RA) and activin, which are candidate regulators of pancreatic development in vivo. These induced tissues had decreased expression of the sonic hedgehog (shh) gene and expressed several pancreatic marker genes. ES cell-derived pancreatic tissue was composed of exocrine cells, endocrine cells, and pancreatic duct-like structures. In addition, the ratio of exocrine to endocrine cells in the induced tissue was found to be sensitive to the concentrations of RA and activin in the present experiment.
体外形成器官和/或组织是再生医学的一个主要目标，这也将为分析每个靶器官的发育机制和疾病过程提供一个强大的工具。在此，我们展示了一种从小鼠胚胎干细胞（ES细胞）体外形成胰腺组织的方法。从ES细胞集落诱导形成的类胚体球体（EBSs）用视黄酸（RA）和激活素处理，它们是体内胰腺发育的候选调节因子。这些诱导组织中声波刺猬因子（shh）基因的表达降低，并表达了几种胰腺标记基因。ES细胞来源的胰腺组织由外分泌细胞、内分泌细胞和胰腺导管样结构组成。此外，在本实验中发现，诱导组织中外分泌细胞与内分泌细胞的比例对RA和激活素的浓度敏感。
