Wosnitza Melanie, Hemmrich Karsten, Groger Andreas, Gräber Steffen, Pallua Norbert
Department of Plastic Surgery and Hand Surgery - Burn Center, University Hospital of the Aachen University of Technology, Pauwelsstr 30, 52057 Aachen, Germany.
Differentiation. 2007 Jan;75(1):12-23. doi: 10.1111/j.1432-0436.2006.00110.x.
Recent research findings postulate that adipocytes and endothelial cells (EC) may share a common progenitor. However, the interlinking pathways between adipose tissue and endothelium, and the differentiation potential of cells to convert from one tissue into the other via progenitor cells have not been elucidated and are therefore the focus of this study. Stromal vascular fraction (SVF) cells were isolated from liposuction aspirates or excised adipose tissue and separated into CD31+ and CD31- populations by magnet-assisted cell sorting. Differentiation to fat tissue was induced in both CD31 fractions after expansion by insulin, dexamethasone, isobutylmethylxanthine, triiodothyronine, pioglitazone, and transferrin. Differentiation was assayed enzymatically and by cell counting. Maturation to endothelium was performed with vascular endothelial growth factor (VEGF), insulin-like growth factor-1 plus 2% fetal calf serum, and confirmed by flow cytometry and tube formation assays on Matrigel. Our results show that the SVF contains a CD31-, S100+ cell type that can differentiate into adipocytes and EC. The SVF also comprises CD31+ cells that, although they have an endothelial phenotype, can be converted into mature adipocytes. These findings demonstrate the potency of SVF cells to perform both adipogenic and endothelial differentiation. Further, they reveal the plasticity of mature cells of mesenchymal origin to undergo conversion from endothelium to adipose tissue and vice versa.
最近的研究结果推测，脂肪细胞和内皮细胞（EC）可能拥有共同的祖细胞。然而，脂肪组织与内皮之间的相互联系途径，以及细胞通过祖细胞从一种组织转化为另一种组织的分化潜能尚未阐明，因此是本研究的重点。从抽脂吸出物或切除的脂肪组织中分离出基质血管组分（SVF）细胞，并通过磁珠辅助细胞分选将其分为CD31 +和CD31-群体。在通过胰岛素、地塞米松、异丁基甲基黄嘌呤、三碘甲状腺原氨酸、吡格列酮和转铁蛋白扩增后，在两个CD31组分中诱导向脂肪组织的分化。通过酶法和细胞计数来测定分化情况。用血管内皮生长因子（VEGF）、胰岛素样生长因子-1加2%胎牛血清使细胞向内皮细胞成熟，并通过流式细胞术和基质胶上的管形成试验进行确认。我们的结果表明，SVF包含一种CD31- S100 +细胞类型，其可分化为脂肪细胞和内皮细胞。SVF还包含CD31 +细胞，尽管它们具有内皮细胞表型，但可转化为成熟脂肪细胞。这些发现证明了SVF细胞具有进行脂肪生成和内皮分化的能力。此外，它们揭示了间充质来源的成熟细胞从内皮细胞转化为脂肪组织以及反之亦然的可塑性。
