Lawson C, Al-Akoum M, Maheux R, Akoum A
Centre de Recherche, Hôpital Saint-François d'Assise, Centre Hospitalier Universitaire de Québec, Québec, Canada.
Reproduction. 2007 Jan;133(1):265-74. doi: 10.1530/rep.1.01121.
The establishment and progression of ectopic endometrial implants are dependent upon their interaction with and responsiveness to the stimuli present in their new environment. According to our and other previous studies, immune cells-derived cytokines, such as IL-1, may alone or in concert with estrogens, enhance the capability of ectopic endometrial cells to implant and develop into the host tissue. In the present study, immunohistochemical and dual immunofluorescence analyses showed that the functional signaling interleukin-1 receptor type 1 (IL-1RI) is expressed in endometriotic tissue, particularly in the glands, and identified endothelial cells, macrophages, and T-lymphocytes as cells having marked expression of IL-1RI. The highest concentrations of IL-1RI protein in endometriotic tissue, as evaluated using histological score (HSCORE) and measured by ELISA, were found in red endometriotic lesions as compared with typical black-blue or white lesions. Western blotting showed a significant increase in the levels of the 50 kDa band, whose apparent molecular weight corresponds to the soluble form of IL-1RI. RT-PCR analysis of IL-1 mRNA levels showed a pattern of expression comparable to that of the protein. Interestingly, IL-1RI expression was more significant in the proliferative than in the secretory phase of the menstrual cycle. Marked expression of IL-1RI, the functional signaling receptor that mediates cell activation by IL-1, in red endometriotic implants, which are highly vascularized and represent the earliest and most active forms of the disease, point to a higher cell receptivity for IL-1 in these lesions, a relationship with the activity of the disease and a possible involvement in the early steps of endometriotic tissue growth and development.
异位子宫内膜植入物的建立和进展取决于它们与新环境中存在的刺激物的相互作用以及对这些刺激物的反应能力。根据我们和其他先前的研究，免疫细胞衍生的细胞因子，如白细胞介素-1（IL-1），可能单独或与雌激素协同作用，增强异位子宫内膜细胞植入并发育成宿主组织的能力。在本研究中，免疫组织化学和双重免疫荧光分析表明，功能性信号转导白细胞介素-1受体1型（IL-1RI）在子宫内膜异位组织中表达，特别是在腺体中，并确定内皮细胞、巨噬细胞和T淋巴细胞为IL-1RI表达明显的细胞。使用组织学评分（HSCORE）评估并通过酶联免疫吸附测定（ELISA）测量发现，与典型的黑蓝色或白色病变相比，红色子宫内膜异位病变中IL-1RI蛋白的浓度最高。蛋白质印迹法显示50 kDa条带的水平显著增加，其表观分子量对应于IL-1RI的可溶性形式。IL-1 mRNA水平的逆转录聚合酶链反应（RT-PCR）分析显示其表达模式与蛋白质的表达模式相当。有趣的是，IL-1RI在月经周期的增殖期比分泌期表达更显著。在高度血管化且代表该疾病最早和最活跃形式的红色子宫内膜异位植入物中，介导IL-1细胞活化的功能性信号转导受体IL-1RI的明显表达表明，这些病变中细胞对IL-1的接受性更高，这与疾病的活性有关，并且可能参与子宫内膜异位组织生长和发育的早期步骤。
