印度南部特里凡得琅分离出的霍乱弧菌O1群埃尔托生物型稻叶血清型菌株的遗传背景特征分析
Characterization of the genetic background of Vibrio cholerae O1 biotype El Tor serotype Inaba strains isolated in Trivandrum, southern India.
作者信息
Mohapatra Saswat S, Ramachandran Dhanya, Mantri Chinmay K, Singh Durg V
机构信息
Institute of Life Sciences, Nalco Square, Bhubaneswar 751 023, India.
Rajiv Gandhi Centre for Biotechnology, Jagathy, Trivandrum 695 014, India.
出版信息
J Med Microbiol. 2007 Feb;56(Pt 2):260-265. doi: 10.1099/jmm.0.46868-0.
Isolates of Vibrio cholerae O1 biotype El Tor serotype Inaba associated with an outbreak of cholera in Trivandrum, southern India, were characterized. PCR testing revealed that all five isolates examined carried the TCP pathogenicity island, the CTX genetic element and the RTX toxin, and produced cholera toxin (CT). RFLP analysis revealed that these Inaba isolates possessed a single copy of the CTX element flanked by two tandemly arranged copies of the RS element upstream of the core region. The isolates were resistant to ampicillin, nalidixic acid, trimethoprim, sulfamethoxazole, streptomycin and the vibriostatic agent 2,4-diamino-6,7-diisopropylpteridine (O/129). Ribotyping of these Inaba isolates revealed a hybridization profile similar to a strain of serotype Ogawa prevalent in southern India.
对与印度南部特里凡得琅霍乱暴发相关的霍乱弧菌O1生物型埃尔托稻叶血清型菌株进行了特征分析。PCR检测显示，所检测的全部5株菌株均携带毒素协同调节菌毛（TCP）致病岛、霍乱毒素基因元件（CTX）和RTX毒素，并产生霍乱毒素（CT）。限制性片段长度多态性（RFLP）分析显示，这些稻叶血清型菌株在核心区域上游拥有单拷贝的CTX元件，两侧为两个串联排列的RS元件拷贝。这些菌株对氨苄西林、萘啶酸、甲氧苄啶、磺胺甲恶唑、链霉素以及弧菌抑制剂2,4-二氨基-6,7-二异丙基蝶啶（O/129）具有抗性。对这些稻叶血清型菌株进行核糖体分型，结果显示其杂交图谱与印度南部流行的一株小川血清型菌株相似。
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