从印度加尔各答住院患者中分离出的近期霍乱弧菌O1型、埃尔托生物型、稻叶菌株的分子特征
Molecular characterization of recent Vibrio cholerae O1, El Tor, Inaba strains isolated from hospitalized patients in Kolkata, India.
作者信息
Raychoudhuri Amit, Chatterjee Souvik, Pazhani Gururaja P, Nandy Ranjan K, Bhattacharya Mihir K, Bhattacharya Sujit K, Ramamurthy T, Mukhopadhyay Asish K
机构信息
National Institute of Cholera and Enteric Diseases, Kolkata, India.
出版信息
J Infect. 2007 Nov;55(5):431-8. doi: 10.1016/j.jinf.2007.07.020. Epub 2007 Sep 12.
OBJECTIVES
To study the phenotypic and genotypic characterization of newly emerged V. cholerae O1, Inaba strains isolated from patients with diarrhoea.
METHODS
Bacterial characterization was made using polymerase chain reaction, ribotyping, PFGE and RFLP.
RESULTS
After its first appearance in July 2004, O1 Inaba became the dominant serotype by March 2005 and totally replaced the former dominant serotype, Ogawa from May 2005. Most of the Inaba isolates belong to a new ribotype RIV. Ogawa and also some Inaba strains isolated during the same period were identified as RIII. Similarly, the majority of the Inaba isolates belong to 'H1' pulsotype and one isolate is type 'H', while the Ogawa isolates were mostly 'H' pulsotype. Presence of CTX prophage was detected in a single site of the chromosome with at least two RS elements.
CONCLUSIONS
There has been a switch of dominant serotype from Ogawa to Inaba in the Kolkata region. This is not necessarily due to emergence of a new clone but does serve as an epidemiological marker. Further analysis at the molecular level will be required to define this trend and to monitor future spread to other regions.
目的
研究从腹泻患者中分离出的新出现的霍乱弧菌O1群稻叶型菌株的表型和基因型特征。
方法
采用聚合酶链反应、核糖体分型、脉冲场凝胶电泳和限制性片段长度多态性进行细菌鉴定。
结果
O1群稻叶型菌株于2004年7月首次出现后，到2005年3月成为优势血清型，并于2005年5月完全取代了之前的优势血清型小川型。大多数稻叶型分离株属于一种新的核糖体分型RIV。小川型以及同期分离出的一些稻叶型菌株被鉴定为RIII。同样，大多数稻叶型分离株属于“H1”脉冲型，一株为“H”型，而小川型分离株大多为“H”脉冲型。在染色体的单个位点检测到霍乱毒素噬菌体，带有至少两个重复序列元件。
结论
加尔各答地区优势血清型已从小川型转变为稻叶型。这不一定是由于新克隆的出现，但确实可作为一种流行病学标志。需要在分子水平上进行进一步分析，以确定这一趋势并监测未来向其他地区的传播。
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