通过原位杂交技术确定果蝇种系 LSP-1 基因的位置。
Location of the LSP-1 Genes in Drosophila Species by IN SITU Hybridization.
机构信息
The Genetics Laboratory, Department of Biochemistry, Oxford University, Oxford, United Kingdom OX1 3QU.
出版信息
Genetics. 1983 Jan;103(1):75-92. doi: 10.1093/genetics/103.1.75.
The locations of the larval serum protein one (LSP-1) alpha, beta and gamma genes were determined in Drosophila melanogaster and in 14 other species of Drosophila by in situ hybridization to polytene chromosomes. The LSP-1 alpha gene mapped to bands 11B on the X chromosome, the LSP-1 beta gene mapped to bands 21D-E on chromosome 2L, and the LSP-1 gamma gene mapped to band 61A in all the melanogaster subgroup species. In eight other species, both the LSP-1 alpha and beta genes mapped to one site on Muller's element E which corresponds to chromosome 3R of D. melanogaster. No hybridization of LSP-1 gamma was detected in these eight species. Restriction enzyme digestion and analysis of genomic DNA by filter transfer hybridization confirmed the presence of LSP-1 alpha-like and beta-like genes in seven of these species. These results are discussed with respect to conservation of the chromosomal elements in the genus Drosophila.
通过对多线染色体的原位杂交，确定了果蝇和其他 14 种果蝇中幼虫血清蛋白 1（LSP-1）的α、β和γ基因的位置。LSP-1α基因定位于 X 染色体的 11B 带，LSP-1β基因定位于 2L 染色体的 21D-E 带，LSP-1γ基因在所有黑腹果蝇亚组物种中定位于 61A 带。在其他 8 种物种中，LSP-1α和β基因都定位于 Muller 元素 E 的一个位点，该位点对应于黑腹果蝇的 3R 染色体。在这 8 个物种中，没有检测到 LSP-1γ的杂交。通过限制性内切酶消化和基因组 DNA 的滤膜转移杂交分析，证实了这 7 个物种中存在 LSP-1α样和β样基因。这些结果与果蝇属染色体元件的保守性有关。
Zotero 插件