Band H T, Band R N, Ives P T
Biochem Genet. 1984 Jun;22(5-6):551-66. doi: 10.1007/BF00484522.
LSP-1 beta S is present in Michigan and Massachusetts Drosophila melanogaster natural populations. Its frequency, 10%, is significantly higher in an East Jordan, Mich. (latitude, 45.10 degrees N), population than in East Lansing, Mich. (latitude 42.44 degrees N), or Hadley, Mass. (latitude, 42.21 degrees N), populations, where it averages 3% at each location. The average frequency of LSP-2S is more comparable, 6, 5, and 7% at East Jordan, East Lansing, and Hadley, respectively. LSP-1 gamma F variants are also present. A total of 342 single third-instar larvae was scored for LSP-1 autosomal variants, and 323 for LSP-2 variants. Each larva represented a newly established isofemale line from collections at East Jordan in 1981 and 1983, East Lansing in 1982, and Hadley in 1981, 1982, and 1983. Within localities, frequencies of hemolymph protein variants did not differ significantly between years. Proteins 9, 10, 11, and 15 correspond to the LSP-1 gamma, beta, and alpha triplet and LSP-2 polypeptide in D. melanogaster. Our results together with those of Singh and Coulthart [(1982). Genetics 102:437] indicate that D. melanogaster populations in north temperate climates maintain considerable genetic heterogeneity for the larval hemolymph proteins.
LSP - 1βS存在于密歇根州和马萨诸塞州的黑腹果蝇自然种群中。其频率为10%，在密歇根州东乔丹（北纬45.10度）的种群中显著高于密歇根州东兰辛（北纬42.44度）或马萨诸塞州哈德利（北纬42.21度）的种群，后两个种群中该频率平均为3%。LSP - 2S的平均频率更具可比性，在东乔丹、东兰辛和哈德利分别为6%、5%和7%。LSP - 1γF变体也存在。共对342只三龄幼虫的LSP - 1常染色体变体进行了评分，对323只幼虫的LSP - 2变体进行了评分。每只幼虫代表一个新建立的同雌系，这些同雌系来自1981年和1983年在东乔丹、1982年在东兰辛以及1981年、1982年和1983年在哈德利采集的样本。在各地点内，血淋巴蛋白变体的频率在不同年份之间没有显著差异。蛋白质9、10、11和15分别对应黑腹果蝇中的LSP - 1γ、β和α三联体以及LSP - 2多肽。我们的结果与辛格和库尔萨特[（1982年）。遗传学102:437]的结果表明，北温带气候下的黑腹果蝇种群在幼虫血淋巴蛋白方面保持着相当大的遗传异质性。
