Pierce D A, Lucchesi J C
Chromosoma. 1980;76(3):245-54. doi: 10.1007/BF00327265.
Four major puffs are inducible by heat shock in the larval salivary gland chromosomes of D. pseudoobscura. Two of these puffs are present at 23 and 39-40 on the right arm of the X chromosome and two are present at 53 and 58 on chromosome 2. By means of in situ hybridization, residual homologies were demonstrated between the puffs at 23 in D. pseudoobscura and at 63 C in D. melanogaster, and between the two chromosome 2 puffs of D. pseudoobscura and 87A and 87C of D. melanogaster. RNA synthesis was monitored as a function of 3H-uridine incorporation in the major heat-induced puffs of D. pseudoobscura and was found to be equivalent in males and females indicating dosage compensation of the two X-linked loci. The evoluation of the regulatory controls of these genes is discussed.
在拟暗果蝇幼虫唾液腺染色体中，热休克可诱导出四个主要的胀泡。其中两个胀泡位于X染色体右臂的23区和39 - 40区，另外两个位于2号染色体的53区和58区。通过原位杂交，证明了拟暗果蝇23区的胀泡与黑腹果蝇63C处的胀泡之间，以及拟暗果蝇2号染色体上的两个胀泡与黑腹果蝇87A和87C之间存在残余同源性。以3H - 尿苷掺入量为指标监测了拟暗果蝇主要热诱导胀泡中的RNA合成，发现雌雄果蝇中该合成量相当，表明两个X连锁基因座存在剂量补偿。本文还讨论了这些基因调控控制的进化。
