单体型四膜虫。二。一组单体型定义了生殖染色体。
Nullisomic Tetrahymena. II. a Set of Nullisomics Define the Germinal Chromosomes.
机构信息
Division of Biological Sciences, Section of Genetics and Development, Cornell University, Ithaca, New York 14853.
出版信息
Genetics. 1983 Jun;104(2):257-70. doi: 10.1093/genetics/104.2.257.
Abstract
Crosses of a diploid Tetrahymena thermophila to a strain with a haploid germinal nucleus result in chromosome loss during meiosis in the haploid. The resulting monosomics can be made nullisomic by a special cross that induces homozygosis of a meiotic product of the germinal nucleus, but retention of the parental somatic nucleus. The creation and testing of single nullisomics for three of the five chromosome pairs and a triple nullisomic missing another pair is presented. Taken together, these strains make possible a series of crosses in which all but one of the chromosomes is missing in one parent. This set of nullisomics can, therefore, be used to map any mutation in Tetrahymena to a specific chromosome.
摘要
二倍体嗜热四膜虫与具有单倍体生殖核的菌株杂交，导致在单倍体减数分裂过程中染色体丢失。通过一种特殊的杂交，可以使由此产生的单体成为缺体，这种杂交会诱导生殖核减数分裂产物的纯合，但保留亲代体细胞核。本文介绍了为五个染色体对中的三个和另一个缺失一对的三单体创造和测试单缺体的情况。总的来说，这些菌株使得一系列杂交成为可能，其中一个亲本的所有染色体除一条外都缺失。因此，这套缺体可以用来将嗜热四膜虫中的任何突变映射到特定的染色体上。
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