National Institute of Genetics, Mishima, 411 Japan.
Genetics. 1984 Feb;106(2):267-77. doi: 10.1093/genetics/106.2.267.
The frequencies of a polymorphic inversion, In(2L)t, and of Adh and alphaGpdh alleles were analyzed in three natural populations of Drosophila melanogaster from Japan. Significant positive correlations between the frequencies of In(2L)t and Adh(S) or alphaGpdh(F) were detected due to tight linkage. An analysis of correlation with latitude showed that the negative cline of Adh(S) frequency could be explained entirely by its linkage with In(2L)t; the frequency of Adh(S) on the standard chromosome did not show a latitudinal cline. To the contrary, the cline of alphaGpdh(F) frequency itself was positive, and its linkage with In(2L)t makes the positive cline unclear. These results suggest that the two allozymes themselves respond to latitudinal natural selection in different ways. When these populations were transferred to laboratory cages and maintained for a long time, they lost the chromosomal polymorphism but retained stable enzyme polymorphisms, although allele frequencies in the cage were not the same as in nature. The frequencies of Adh and alphaGpdh alleles were close to those in earlier cage populations of the same geographical origin.
在来自日本的三个黑腹果蝇自然种群中，分析了一个多态倒位（In(2L)t）和 Adh 和 alphaGpdh 等位基因的频率。由于紧密连锁，检测到 In(2L)t 和 Adh(S)或 alphaGpdh(F)频率之间存在显著的正相关。与纬度的相关分析表明，Adh(S)频率的负向梯度完全可以用其与 In(2L)t 的连锁来解释；标准染色体上 Adh(S)的频率没有表现出纬度梯度。相反，alphaGpdh(F)频率本身的梯度是正向的，而其与 In(2L)t 的连锁使得正向梯度不清晰。这些结果表明，这两个同工酶本身以不同的方式对纬度自然选择做出反应。当这些种群被转移到实验室笼中并长期维持时，它们失去了染色体多态性，但保留了稳定的酶多态性，尽管笼中的等位基因频率与自然界不同。Adh 和 alphaGpdh 等位基因的频率接近同一地理起源的早期笼养种群的频率。
