Laboratoire de Biologie cellulaire, Institut National de la Recherche Agronomique, F 78000 Versailles.
Genetics. 1985 Feb;109(2):393-407. doi: 10.1093/genetics/109.2.393.
In previous experiments, seven lines of valine-resistant plants were regenerated from protoplast-derived haploid tobacco mesophyll cells which had been UV mutagenized and submitted to selection by toxic concentrations of valine. In this study we described the transmission of valine-resistance to progeny and a preliminary phenotypical and biochemical characterization of the resistant plants.-Two types were thus distinguished among the seven mutant lines. Valine-resistance of the mutants of the first type (three lines) was transmitted as a single Mendelian dominant character (Vr1), whereas valine-resistance of the second type (four lines) was transmitted as a digenic recessive character (vr2 and vr3). Allelism tests revealed that the four recessive mutant lines yielded resistant progeny when intercrossed and, therefore, bear recessive mutant alleles at the same two unlinked loci.-When cultured at a density of 100 cell/ml, protoplast-derived cells of mutants of the first type had a low level of resistance to valine, whereas protoplast-derived cells of mutants of the second type displayed a high level of resistance to valine and to other amino acids.-According to the results of (14)C-labelled amino acid uptake experiments, the amino acid resistance of mutants of the second type, but not valine-resistance of the first type, could be accounted for by reduced uptake of several amino acids. Possible uses of valine-resistance as a marker in plant cell genetics are discussed.
在以前的实验中，已从经 UV 诱变和用毒性浓度缬氨酸进行选择的原生质体衍生的烟草叶肉细胞中再生了 7 株缬氨酸抗性植株。在这项研究中，我们描述了缬氨酸抗性向后代的传递，以及对抗性植株的初步表型和生化特征的描述。-在这 7 条突变株系中，两种类型的缬氨酸抗性被区分开来。第一类（3 个株系）突变株的缬氨酸抗性作为一个单一的孟德尔显性特征（Vr1）传递，而第二类（4 个株系）的缬氨酸抗性作为双基因隐性特征（vr2 和 vr3）传递。等位基因测试表明，第二类型的四个隐性突变株系在相互杂交时产生抗性后代，因此，在两个不相关的基因座上携带隐性突变等位基因。-当以 100 个细胞/ml 的密度培养时，第一类型突变株的原生质体衍生细胞对缬氨酸的抗性水平较低，而第二类型突变株的原生质体衍生细胞对缬氨酸和其他氨基酸的抗性水平较高。-根据 14C 标记氨基酸摄取实验的结果，第二类型突变株的氨基酸抗性，但不是第一类型突变株的缬氨酸抗性，可以用几种氨基酸摄取减少来解释。讨论了将缬氨酸抗性作为植物细胞遗传学中的标记的可能用途。
