Suppr超能文献

新型线虫琥珀抑制物。

Novel nematode amber suppressors.

机构信息

MRC Laboratory of Molecular Biology, Cambridge CB2 2QH, England.

出版信息

Genetics. 1985 Oct;111(2):287-310. doi: 10.1093/genetics/111.2.287.

Abstract

Nine amber suppressor mutations were isolated in the nematode Caenorhabditis elegans by reverting amber alleles of a sex-determining gene, tra-3. One suppressor maps to a known locus, sup-5 III , but the other eight map to three new loci, sup-21 X (five alleles), sup-22 IV (two alleles) and sup-23 IV (one allele). Amber alleles of tra-3 and of a dumpy gene, dpy-20, were used to measure the efficiency of suppression; the sup-21 and the sup-22 alleles were both shown to be heterogeneous and generally weaker suppressors than sup-5 alleles, which are homogeneous. The spectrum of mutations suppressed by a strong sup-21 allele, e1957, was investigated and compared to the spectra for the amber suppressors sup-5 III and sup-7 X, using amber alleles in 13 assorted genes. Some of the differences between these spectra may be due to limited tissue specificity in sup-21 expression.-Suppression of dpy-20 was used to show that the sex-linked suppressors sup-7 and sup-21 are not dosage compensated in male (XO) relative to hermaphrodite (XX).-Several uses of amber suppressors are critically discussed: for identifying null mutations, for varying levels of gene activity and for detecting maternal mRNA.

摘要

通过回复性转变基因 tra-3 的琥珀色等位基因,在秀丽隐杆线虫中分离出 9 个琥珀色抑制突变。一个抑制子映射到一个已知的位置 sup-5 III ,但其他 8 个映射到三个新的位置 sup-21 X (5 个等位基因)、sup-22 IV (2 个等位基因)和 sup-23 IV (1 个等位基因)。tra-3 和矮胖基因 dpy-20 的琥珀色等位基因被用来测量抑制效率;sup-21 和 sup-22 等位基因都显示出异质性,通常比 sup-5 等位基因弱,而 sup-5 等位基因是同质的。使用 13 个不同基因中的琥珀色等位基因,研究了强 sup-21 等位基因 e1957 抑制的突变谱,并与琥珀色抑制子 sup-5 III 和 sup-7 X 的谱进行了比较。这些谱之间的一些差异可能是由于 sup-21 表达的组织特异性有限。-抑制 dpy-20 被用来表明性连锁抑制子 sup-7 和 sup-21 在雄性(XO)相对于雌雄同体(XX)中没有剂量补偿。-几个琥珀色抑制子的用途受到了严格的讨论:用于鉴定缺失突变、用于改变基因活性水平和用于检测母体 mRNA。

相似文献

1
Novel nematode amber suppressors.
Genetics. 1985 Oct;111(2):287-310. doi: 10.1093/genetics/111.2.287.
5
Differential expression of five tRNA(UAGTrp) amber suppressors in Caenorhabditis elegans.
Mol Cell Biol. 1988 Sep;8(9):3627-35. doi: 10.1128/mcb.8.9.3627-3635.1988.
6
Genetic and molecular analysis of eight tRNA(Trp) amber suppressors in Caenorhabditis elegans.
J Mol Biol. 1990 Sep 5;215(1):7-19. doi: 10.1016/S0022-2836(05)80090-7.
8
A new kind of informational suppression in the nematode Caenorhabditis elegans.
Genetics. 1989 Oct;123(2):301-13. doi: 10.1093/genetics/123.2.301.

引用本文的文献

1
Applications of genetic code expansion technology in eukaryotes.
Protein Cell. 2024 May 7;15(5):331-363. doi: 10.1093/procel/pwad051.
3
Genetic Code Expansion in Animals.
ACS Chem Biol. 2018 Sep 21;13(9):2375-2386. doi: 10.1021/acschembio.8b00520. Epub 2018 Sep 5.
4
Tissue- and Time-Specific Expression of Otherwise Identical tRNA Genes.
PLoS Genet. 2016 Aug 25;12(8):e1006264. doi: 10.1371/journal.pgen.1006264. eCollection 2016 Aug.
5
Expanding the genetic code of Caenorhabditis elegans using bacterial aminoacyl-tRNA synthetase/tRNA pairs.
ACS Chem Biol. 2012 Jul 20;7(7):1292-302. doi: 10.1021/cb200542j. Epub 2012 May 11.
6
Expanding the genetic code of an animal.
J Am Chem Soc. 2011 Sep 14;133(36):14196-9. doi: 10.1021/ja2054034. Epub 2011 Aug 22.
7
A Caenorhabditis elegans wild type defies the temperature-size rule owing to a single nucleotide polymorphism in tra-3.
PLoS Genet. 2007 Mar 2;3(3):e34. doi: 10.1371/journal.pgen.0030034. Epub 2007 Jan 9.
8
Integrative transformation of Caenorhabditis elegans.
EMBO J. 1986 Oct;5(10):2673-80. doi: 10.1002/j.1460-2075.1986.tb04550.x.
9
Natural variation and copulatory plug formation in Caenorhabditis elegans.
Genetics. 1997 May;146(1):149-64. doi: 10.1093/genetics/146.1.149.

本文引用的文献

1
Formaldehyde mutagenesis in the nematode Caenorhabditis elegans.
Mutat Res. 1981 Feb;80(2):273-9. doi: 10.1016/0027-5107(81)90100-7.
2
Mutants with altered muscle structure of Caenorhabditis elegans.
Dev Biol. 1980 Jun 15;77(2):271-302. doi: 10.1016/0012-1606(80)90475-3.
3
Suppression of an amber mutation by microinjection of suppressor tRNA in C. elegans.
Nature. 1982 Sep 30;299(5882):456-8. doi: 10.1038/299456a0.
4
Two types of sex determination in a nematode.
Nature. 1983;304(5923):267-8. doi: 10.1038/304267a0.
5
Dosage compensation of serine-4 transfer RNA in Drosophila melanogaster.
Genetics. 1982 Nov;102(3):525-37. doi: 10.1093/genetics/102.3.525.
6
Correspondence of yeast UAA suppressors to cloned tRNASerUCA genes.
J Mol Biol. 1981 Aug 15;150(3):375-87. doi: 10.1016/0022-2836(81)90553-2.
9
The sup-7(st5) X gene of Caenorhabditis elegans encodes a tRNATrpUAG amber suppressor.
Proc Natl Acad Sci U S A. 1984 Nov;81(21):6784-8. doi: 10.1073/pnas.81.21.6784.
10
Gene regulation for higher cells: a theory.
Science. 1969 Jul 25;165(3891):349-57. doi: 10.1126/science.165.3891.349.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验